Key features and details
- Rabbit monoclonal [EPR8285] to GBP1 - BSA and Azide free
- Suitable for: ICC/IF, IHC-P, WB
- Knockout validated
- Reacts with: Human
Product nameAnti-GBP1 antibody [EPR8285] - BSA and Azide free
See all GBP1 primary antibodies
DescriptionRabbit monoclonal [EPR8285] to GBP1 - BSA and Azide free
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide within Human GBP1 aa 550-650. The exact sequence is proprietary.
- WB: A549 and HeLa (Untreated or treated with 10ng/ml IFN-? for 24 hours) cell lysates. ICC/IF: IFN-? induced HeLa cells. IHC-P: Human spleen tissue.
ab240050 is the carrier-free version of ab131255 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Ab240050 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Dissociation constant (KD)KD = 2.00 x 10 -12 M Learn more about KD
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab240050 in the following tested applications.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).|
FunctionBinds GTP, GDP and GMP.
Sequence similaritiesBelongs to the GBP family.
Cellular localizationCell membrane.
- Information by UniProt
- GBP 1 antibody
- GBP-1 antibody
- GBP1 antibody
All lanes : Anti-GBP1 antibody [EPR8285] (ab131255) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : GBP1 knockout A549 cell lysate
Lane 3 : HeLa treated with 10ng/ml IFN-? for 24 hours, whole cell lysate
Lane 4 : Untreated HeLa cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
This data was developed using the same antibody clone in a different buffer formulation (ab131255).
ab131255 Anti-GBP1 antibody [EPR8285] was shown to specifically react with GBP1 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267202 (knockout cell lysate ab257960) was used. Wild-type and GBP1 knockout samples were subjected to SDS-PAGE. ab131255 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling GBP1 with ab131255 antibody at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131255).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab240050 has not yet been referenced specifically in any publications.