Anti-GBP2 antibody (ab203238)
Key features and details
- Rabbit polyclonal to GBP2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GBP2 antibody
See all GBP2 primary antibodies -
Description
Rabbit polyclonal to GBP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Mouse GBP2 aa 150-250 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9Z0E6 -
Positive control
- WB: Mouse lung tissue lysate. IHC-P: Human colon cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100 - 1/1000. Predicted molecular weight: 67 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/100 - 1/1000. Predicted molecular weight: 67 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Guanylate-binding proteins (GBPs) are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP). GBP2 is a GTPase that converts GTP to GDP and GMP. -
Cellular localization
Cell membrane; Lipid-anchor; Cytoplasmic side -
Database links
- Entrez Gene: 2634 Human
- Entrez Gene: 14469 Mouse
- Entrez Gene: 171164 Rat
- Omim: 600412 Human
- SwissProt: P32456 Human
- SwissProt: Q9Z0E6 Mouse
- SwissProt: Q63663 Rat
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Alternative names
- GBP 2 antibody
- GTP binding protein 2 antibody
- Guanine nucleotide binding protein 2 antibody
see all
Images
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Anti-GBP2 antibody (ab203238) at 1/300 dilution + Mouse lung lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/500 dilution
Predicted band size: 67 kDa -
Immunohistochemical analysis of formalin-fixed paraffin-embedded human colon cancer tissue labeling GBP2 with ab203238 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab203238 has been referenced in 5 publications.
- Wan T et al. Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice. Nat Commun 13:1134 (2022). PubMed: 35241660
- Cao S et al. Subversion of GBP-mediated host defense by E3 ligases acquired during Yersinia pestis evolution. Nat Commun 13:4526 (2022). PubMed: 35927280
- Zhu X et al. Impact of Aβ40 and Aβ42 Fibrils on the Transcriptome of Primary Astrocytes and Microglia. Biomedicines 10:N/A (2022). PubMed: 36428550
- Lu Y et al. Neuron-Derived Estrogen Is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain. J Neurosci 40:7355-7374 (2020). PubMed: 32817249
- Wang J et al. Guanylate-binding protein-2 inhibits colorectal cancer cell growth and increases the sensitivity to paclitaxel of paclitaxel-resistant colorectal cancer cells by interfering Wnt signaling. J Cell Biochem 121:1250-1259 (2020). PubMed: 31489998