Key features and details
- Rabbit polyclonal to GBP2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GBP2 antibody
See all GBP2 primary antibodies
DescriptionRabbit polyclonal to GBP2
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human urinary bladder tissue. ICC/IF: RT-4 cells. WB: RT-4 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab247040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceGuanylate-binding proteins (GBPs) are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP). GBP2 is a GTPase that converts GTP to GDP and GMP.
Cellular localizationCell membrane; Lipid-anchor; Cytoplasmic side
- GBP 2 antibody
- GTP binding protein 2 antibody
- Guanine nucleotide binding protein 2 antibody
PFA fixed, Triton X-100 permeabilized RT4 (Human urinary bladder cancer cell line) cells labeling GBP2 using ab247040 at 4 µg/ml (green) in ICC/IF.
Paraffin-embedded human urinary bladder tissue stained for GBP2 using ab247040 at 1/50 dilution in immunohistochemical analysis.
Anti-GBP2 antibody (ab247040) at 0.4 µg/ml + RT4 (Human urinary bladder cancer cell line) cell lysate
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab247040 has not yet been referenced specifically in any publications.