Overview

  • Product name

    Anti-GC-1 antibody
  • Description

    Rabbit polyclonal to GC-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to Human GC-1 aa 1-323.

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji cell lysates. Mouse brain; A431 cells; OVCAR3 xenograft tissue
  • General notes

    Previously labelled as SLC25A22.

Properties

Applications

Our Abpromise guarantee covers the use of ab137614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Involved in the transport of glutamate across the inner mitochondrial membrane. Glutamate is cotransported with H(+).
  • Tissue specificity

    Highly expressed in most tissues.
  • Involvement in disease

    Defects in SLC25A22 are the cause of epileptic encephalopathy early infantile type 3 (EIEE3) [MIM:609304]; also known as early myoclonic encephalopathy (EME) or neonatal epilepsy with suppression-burst pattern. Severe neonatal epilepsies with suppression-burst pattern are early-onset epileptic syndromes characterized by a typical EEG pattern. The suppression-burst pattern of the EEG is characterized by higher-voltage bursts of slow waves mixed with multifocal spikes alternating with isoelectric suppression phases. EME is characterized by a very early onset, erratic and fragmentary myoclonus, massive myoclonus, partial motor seizures and late tonic spasms. The prognosis of EME is poor, with no effective treatment, and children with the condition either die within 1 to 2 years after birth or survive in a persistent vegetative state. EME inheritance is autosomal recessive.
  • Sequence similarities

    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GC 1 antibody
    • GC-1 antibody
    • GC1 antibody
    • GHC1_HUMAN antibody
    • Glutamate/H(+) symporter 1 antibody
    • Mitochondrial glutamate carrier 1 antibody
    • SLC25A22 antibody
    • Solute carrier family 25 member 22 antibody
    see all

Images

  • All lanes : Anti-GC-1 antibody (ab137614) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 34 kDa



    10% SDS PAGE
  • Anti-GC-1 antibody (ab137614) at 1/10000 dilution + Mouse brain whole cell lysate at 20 µg

    Predicted band size: 34 kDa



    10% SDS PAGE
  • ab137614 at 1/200 dilution staining GC-1 in paraformaldehyde fixed A431 cells by Immunofluorescence. Lower image merged with DNA probe.

  • ab137614 at 1/500 dilution, staining GC-1 in paraffin embedded OVCAR3 xenograft tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Chen MW & Wu XJ SLC25A22 Promotes Proliferation and Metastasis of Osteosarcoma Cells via the PTEN Signaling Pathway. Technol Cancer Res Treat 17:1533033818811143 (2018). Read more (PubMed: 30482097) »
  • Kang Y  et al. Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex. Mol Cell 67:457-470.e5 (2017). Read more (PubMed: 28712726) »
See all 3 Publications for this product

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