Anti-GC-1 antibody (ab137614)
Key features and details
- Rabbit polyclonal to GC-1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GC-1 antibody -
Description
Rabbit polyclonal to GC-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to Human GC-1 aa 1-323.
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Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji cell lysates. Mouse brain; A431 cells; OVCAR3 xenograft tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 34 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Involved in the transport of glutamate across the inner mitochondrial membrane. Glutamate is cotransported with H(+). -
Tissue specificity
Highly expressed in most tissues. -
Involvement in disease
Defects in SLC25A22 are the cause of epileptic encephalopathy early infantile type 3 (EIEE3) [MIM:609304]; also known as early myoclonic encephalopathy (EME) or neonatal epilepsy with suppression-burst pattern. Severe neonatal epilepsies with suppression-burst pattern are early-onset epileptic syndromes characterized by a typical EEG pattern. The suppression-burst pattern of the EEG is characterized by higher-voltage bursts of slow waves mixed with multifocal spikes alternating with isoelectric suppression phases. EME is characterized by a very early onset, erratic and fragmentary myoclonus, massive myoclonus, partial motor seizures and late tonic spasms. The prognosis of EME is poor, with no effective treatment, and children with the condition either die within 1 to 2 years after birth or survive in a persistent vegetative state. EME inheritance is autosomal recessive. -
Sequence similarities
Belongs to the mitochondrial carrier family.
Contains 3 Solcar repeats. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 504371 Cow
- Entrez Gene: 79751 Human
- Entrez Gene: 68267 Mouse
- Omim: 609302 Human
- SwissProt: Q08DK4 Cow
- SwissProt: Q9H936 Human
- SwissProt: Q9D6M3 Mouse
- Unigene: 99486 Human
see all -
Alternative names
- GC 1 antibody
- GC-1 antibody
- GC1 antibody
see all
Images
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All lanes : Anti-GC-1 antibody (ab137614) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDa
10% SDS PAGE -
Anti-GC-1 antibody (ab137614) at 1/10000 dilution + Mouse brain whole cell lysate at 20 µg
Predicted band size: 34 kDa
10% SDS PAGE -
ab137614 at 1/200 dilution staining GC-1 in paraformaldehyde fixed A431 cells by Immunofluorescence. Lower image merged with DNA probe.
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ab137614 at 1/500 dilution, staining GC-1 in paraffin embedded OVCAR3 xenograft tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab137614 has been referenced in 4 publications.
- Kang Y et al. Function of hTim8a in complex IV assembly in neuronal cells provides insight into pathomechanism underlying Mohr-Tranebjærg syndrome. Elife 8:N/A (2019). PubMed: 31682224
- Chen MW & Wu XJ SLC25A22 Promotes Proliferation and Metastasis of Osteosarcoma Cells via the PTEN Signaling Pathway. Technol Cancer Res Treat 17:1533033818811143 (2018). PubMed: 30482097
- Kang Y et al. Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex. Mol Cell 67:457-470.e5 (2017). PubMed: 28712726
- Kang Y et al. Tim29 is a novel subunit of the human TIM22 translocase and is involved in complex assembly and stability. Elife 5:N/A (2016). PubMed: 27554484