Recombinant
RabMAb

Recombinant Anti-GC-C antibody [EPR22056-278] (ab225864)

Overview

  • Product name

    Anti-GC-C antibody [EPR22056-278]
    See all GC-C primary antibodies
  • Description

    Rabbit monoclonal [EPR22056-278] to GC-C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Mouse GC-C aa 1-450. The exact sequence is proprietary.
    Database link: Q3UWA6

  • Positive control

    • WB: Mouse spleen and colon lysates; Rat colon lysate. IHC-P: Mouse and rat colon tissues.
  • General notes

    Previously labelled as GUCY2C. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22056-278
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab225864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 145, 130 kDa (predicted molecular weight: 123 kDa).

Target

  • Function

    Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin.
  • Sequence similarities

    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
    Contains 1 guanylate cyclase domain.
    Contains 1 protein kinase domain.
  • Domain

    The protein kinase domain is predicted to be catalytically inactive.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GC-C antibody
    • Guanylyl cyclase C antibody
    • GUC2C antibody
    • GUC2C_HUMAN antibody
    • GUCY2C antibody
    • Heat stable enterotoxin receptor antibody
    • Heat-stable enterotoxin receptor antibody
    • hSTAR antibody
    • Intestinal guanylate cyclase antibody
    • STA receptor antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling GC-C with ab225864 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in epithelium of mouse colon (PMID: 27246004) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • All lanes : Anti-GC-C antibody [EPR22056-278] (ab225864) at 1/1000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Mouse colon lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 123 kDa
    Observed band size: 145,90 kDa
    why is the actual band size different from the predicted?



    Exposure time: Lane 1: 3 minutes; Lane 2: 26 seconds.

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    GC-C migrates as a glycosylated 145 kDa protein and a 90 kDa cleavage product (PMID: 9344852; PMID: 26986842).

    Negative control: mouse spleen.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling GC-C with ab225864 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in epithelium of rat colon (PMID: 27246004) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GC-C with ab225864 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin.

    Negative control: no staining on mouse spleen (PMID: 26986842) is observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-GC-C antibody [EPR22056-278] (ab225864) at 1/1000 dilution + Rat colon lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 123 kDa
    Observed band size: 130,145 kDa why is the actual band size different from the predicted?


    Exposure time: 37 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    GC-C migrates as two differentially glycosylated proteins with molecular masses of 130 and 145 kDa (PMID: 24217248).

References

ab225864 has not yet been referenced specifically in any publications.

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