Product nameAnti-GC1q R antibody
See all GC1q R primary antibodies
DescriptionRabbit polyclonal to GC1q R
Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Chimpanzee, Gorilla, Orangutan, Bat
Immunogen corresponds to a region within amino acids 150-200 of Human GC1q R (NP_001203.1)
- HeLa, 293T and NIH3T3 whole cells lysate IF/ICC: HeLa cell line
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab101267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 31 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionBinds to the globular "heads" of C1Q thus inhibiting C1 activation.
Sequence similaritiesBelongs to the MAM33 family.
Cellular localizationMitochondrion matrix. Nucleus. Might also be nuclear in some cell types.
- Information by UniProt
- ASF/SF2 associated protein p32 antibody
- C1q globular domain binding protein antibody
- C1qBP antibody
All lanes : Anti-GC1q R antibody (ab101267) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg/ml
Lane 2 : HeLa whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 5 µg/ml
Lane 4 : 293T whole cell lysate at 50 µg/ml
Lane 5 : NIH3T3 whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 31 kDa
Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling GC1q R with ab101267 at 1/1000 (1µg/ml).
ICC/IF image of ab101267 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101267, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Detection of Human GC1q R in 1mg HeLa whole cell lysate (20% of IP loaded) using ab101267 at 3µg/mg of lysate. Further Western Blot detection was performed using ab101267 at 0.4µg/ml.
Lane 1: ab101267 IP.
Lane 2: control IgG IP.
ab101267 has not yet been referenced specifically in any publications.