Key features and details
- Rabbit polyclonal to GC1q R
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-GC1q R antibody
See all GC1q R primary antibodies
DescriptionRabbit polyclonal to GC1q R
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Chimpanzee, Gorilla, Orangutan, Bat
Immunogen corresponds to a region within amino acids 150-200 of Human GC1q R (NP_001203.1)
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab101267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 31 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionBinds to the globular "heads" of C1Q thus inhibiting C1 activation.
Sequence similaritiesBelongs to the MAM33 family.
Cellular localizationMitochondrion matrix. Nucleus. Might also be nuclear in some cell types.
- Information by UniProt
- ASF/SF2 associated protein p32 antibody
- C1q globular domain binding protein antibody
- C1qBP antibody
All lanes : Anti-GC1q R antibody (ab101267) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg/ml
Lane 2 : HeLa whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 5 µg/ml
Lane 4 : 293T whole cell lysate at 50 µg/ml
Lane 5 : NIH3T3 whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 31 kDa
Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling GC1q R with ab101267 at 1/1000 (1µg/ml).
Detection of Human GC1q R in 1mg HeLa whole cell lysate (20% of IP loaded) using ab101267 at 3µg/mg of lysate. Further Western Blot detection was performed using ab101267 at 0.4µg/ml.
Lane 1: ab101267 IP.
Lane 2: control IgG IP.
ab101267 has not yet been referenced specifically in any publications.