Key features and details
- Rabbit polyclonal to GC1q R
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GC1q R antibody
See all GC1q R primary antibodies
DescriptionRabbit polyclonal to GC1q R
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human GC1q R aa 50-100. The exact sequence is proprietary. (NP_001203.1).
Database link: Q07021
- WB: HeLa whole cell lysate. IHC-P: Human lung carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225621 was affinity purified using an epitope specific to GC1q R immobilized on solid support.
Our Abpromise guarantee covers the use of ab225621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 31 kDa.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionBinds to the globular "heads" of C1Q thus inhibiting C1 activation.
Sequence similaritiesBelongs to the MAM33 family.
Cellular localizationMitochondrion matrix. Nucleus. Might also be nuclear in some cell types.
- Information by UniProt
- ASF/SF2 associated protein p32 antibody
- C1q globular domain binding protein antibody
- C1qBP antibody
All lanes : Anti-GC1q R antibody (ab225621) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 31 kDa
Exposure time: 10 seconds
Formalin fixed, paraffin embedded human lung carcinoma tissue stained for GC1q R with ab225621 at 1/200 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225621 has not yet been referenced specifically in any publications.