Overview

  • Product name

  • Description

    Rabbit polyclonal to GC1q R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GC1q R aa 50-100. The exact sequence is proprietary. (NP_001203.1).
    Database link: Q07021

  • Positive control

    • WB: HeLa whole cell lysate. IHC-P: Human lung carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab225621 was affinity purified using an epitope specific to GC1q R immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab225621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Binds to the globular "heads" of C1Q thus inhibiting C1 activation.
    • Sequence similarities

      Belongs to the MAM33 family.
    • Cellular localization

      Mitochondrion matrix. Nucleus. Might also be nuclear in some cell types.
    • Information by UniProt
    • Database links

    • Alternative names

      • ASF/SF2 associated protein p32 antibody
      • C1q globular domain binding protein antibody
      • C1qBP antibody
      • C1QBP_HUMAN antibody
      • Complement component 1 q subcomponent binding protein antibody
      • Complement component 1 Q subcomponent binding protein mitochondrial antibody
      • Complement component 1 Q subcomponent-binding protein, mitochondrial antibody
      • GC1Q R antibody
      • GC1q R protein antibody
      • GC1q-R protein antibody
      • GC1QBP antibody
      • GC1QR antibody
      • globular domain of, C1q, receptor for antibody
      • Glycoprotein gC1qBP antibody
      • HABP 1 antibody
      • HABP1 antibody
      • Hyaluronan binding protein 1 antibody
      • Hyaluronan-binding protein 1 antibody
      • Mitochondrial matrix protein p32 antibody
      • p32 antibody
      • p32 splicing factor antibody
      • p33 antibody
      • Pre mrna splicing factor SF2 P32 subunit precursor antibody
      • SF2p32 antibody
      • Splicing factor SF2 associated protein antibody
      see all

    Images

    • All lanes : Anti-GC1q R antibody (ab225621) at 0.04 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
      Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
      Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 31 kDa


      Exposure time: 10 seconds
    • Formalin fixed, paraffin embedded human lung carcinoma tissue stained for GC1q R with ab225621 at 1/200 dilution in immunohistochemical analysis.

    References

    ab225621 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab225621.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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