Anti-GC1q R antibody (ab232712)
Key features and details
- Rabbit polyclonal to GC1q R
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GC1q R antibody
See all GC1q R primary antibodies -
Description
Rabbit polyclonal to GC1q R -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment (His-tag) corresponding to Human GC1q R aa 50 to the C-terminus. (Expressed in E.coli).
Database link: Q07021 -
Positive control
- IHC-P: Human lung cancer, prostate gland cancer, kidney and rectum cancer tissues. ICC/IF: HeLa cells. WB: K562 and HeLa whole cell lysate (ab150035); Human liver lysate; Recombinant human GC1q R protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab232712 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232712 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 - 20 µg/ml.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 31 kDa.
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Notes |
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ICC/IF
Use a concentration of 5 - 20 µg/ml. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 31 kDa. |
Target
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Function
Binds to the globular "heads" of C1Q thus inhibiting C1 activation. -
Sequence similarities
Belongs to the MAM33 family. -
Cellular localization
Mitochondrion matrix. Nucleus. Might also be nuclear in some cell types. - Information by UniProt
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Database links
- Entrez Gene: 708 Human
- Entrez Gene: 12261 Mouse
- Entrez Gene: 29681 Rat
- Omim: 601269 Human
- SwissProt: Q07021 Human
- SwissProt: O35658 Mouse
- SwissProt: O35796 Rat
- Unigene: 555866 Human
see all -
Alternative names
- ASF/SF2 associated protein p32 antibody
- C1q globular domain binding protein antibody
- C1qBP antibody
see all
Images
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Anti-GC1q R antibody (ab232712) at 3 µg/ml + Recombinant human GC1q R protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 31 kDa -
Paraffin-embedded human lung cancer tissue stained for GC1q R using ab232712 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human prostate gland cancer tissue stained for GC1q R using ab232712 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human kidney tissue stained for GC1q R using ab232712 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human rectum cancer tissue stained for GC1q R using ab232712 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GC1q R (green) using ab232712 at 20 µg/ml in ICC/IF, followed FITC staining.
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All lanes : Anti-GC1q R antibody (ab232712) at 3 µg/ml
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : HeLa (epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Human liver lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 31 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab232712 has not yet been referenced specifically in any publications.