Key features and details
- Rabbit polyclonal to GC1q R
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GC1q R antibody
See all GC1q R primary antibodies
DescriptionRabbit polyclonal to GC1q R
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment (His-tag) corresponding to Human GC1q R aa 76-282. (Expressed in E.coli).
TDGDKAFVDFLSDEIKEERKIQKHKTLPKMSGGWELELNGTEAKLVRKVA GEKITVTFNINNSIPPTFDGEEEPSQGQKVEEQEPELTSTPNFVVEVIKN DDGKKALVLDCHYPEDEVGQEDEAESDIFSIREVSFQSTGESEWKDTNYT LNTDSLDWALYDHLMDFLADRGVDNTFADELVELSTALEHQEYITFLEDL KSFVKSQ
Database link: Q07021
- IHC-P: Human lung cancer, prostate gland cancer, kidney and rectum cancer tissues. ICC/IF: HeLa cells. WB: K562 and HeLa whole cell lysate (ab150035); Human liver lysate; Recombinant human GC1q R protein.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232712 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232712 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 31 kDa.|
FunctionBinds to the globular "heads" of C1Q thus inhibiting C1 activation.
Sequence similaritiesBelongs to the MAM33 family.
Cellular localizationMitochondrion matrix. Nucleus. Might also be nuclear in some cell types.
- Information by UniProt
- ASF/SF2 associated protein p32 antibody
- C1q globular domain binding protein antibody
- C1qBP antibody
Anti-GC1q R antibody (ab232712) at 3 µg/ml + Recombinant human GC1q R protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 31 kDa
Paraffin-embedded human lung cancer tissue stained for GC1q R using ab232712 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human prostate gland cancer tissue stained for GC1q R using ab232712 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human kidney tissue stained for GC1q R using ab232712 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human rectum cancer tissue stained for GC1q R using ab232712 at 20 µg/ml in immunohistochemical analysis. DAB staining.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GC1q R (green) using ab232712 at 20 µg/ml in ICC/IF, followed FITC staining.
All lanes : Anti-GC1q R antibody (ab232712) at 3 µg/ml
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : HeLa (epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Human liver lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 31 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232712 has not yet been referenced specifically in any publications.