Overview

  • Product name

    Anti-GC1q R antibody [EPR8870]
    See all GC1q R primary antibodies
  • Description

    Rabbit monoclonal [EPR8870] to GC1q R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GC1q R aa 150-250. The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, Jurkat and Raji cell lysates. Human tonsil and colon tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Binds to the globular "heads" of C1Q thus inhibiting C1 activation.
    • Sequence similarities

      Belongs to the MAM33 family.
    • Cellular localization

      Mitochondrion matrix. Nucleus. Might also be nuclear in some cell types.
    • Information by UniProt
    • Database links

    • Alternative names

      • ASF/SF2 associated protein p32 antibody
      • C1q globular domain binding protein antibody
      • C1qBP antibody
      • C1QBP_HUMAN antibody
      • Complement component 1 q subcomponent binding protein antibody
      • Complement component 1 Q subcomponent binding protein mitochondrial antibody
      • Complement component 1 Q subcomponent-binding protein, mitochondrial antibody
      • GC1Q R antibody
      • GC1q R protein antibody
      • GC1q-R protein antibody
      • GC1QBP antibody
      • GC1QR antibody
      • globular domain of, C1q, receptor for antibody
      • Glycoprotein gC1qBP antibody
      • HABP 1 antibody
      • HABP1 antibody
      • Hyaluronan binding protein 1 antibody
      • Hyaluronan-binding protein 1 antibody
      • Mitochondrial matrix protein p32 antibody
      • p32 antibody
      • p32 splicing factor antibody
      • p33 antibody
      • Pre mrna splicing factor SF2 P32 subunit precursor antibody
      • SF2p32 antibody
      • Splicing factor SF2 associated protein antibody
      see all

    Images

    • All lanes : Anti-GC1q R antibody [EPR8870] (ab134926) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 31 kDa

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling GC1q R with ab134926 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling GC1q R with ab134926 at 1/100 dilution.
    • Immunohistochemical analysis using ab134926 showing positive staining in Normal breast tissue.

    • Immunohistochemical analysis using ab134926 showing positive staining in Normal uterus tissue.

    • Immunohistochemical analysis using ab134926 showing positive staining in Normal ovary tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab134926 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Placenta)
    Gel Running Conditions
    Reduced Denaturing (12,5%)
    Loading amount
    50 µg
    Specification
    Placenta
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Dr. Polina Vishnyakova

    Verified customer

    Submitted Mar 29 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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