Recombinant Anti-GC1q R antibody [EPR8871] (ab131284)
![Western blot - Anti-GC1q R antibody [EPR8871] (ab131284)](https://www.abcam.com/ps/products/131/ab131284/Images/ab131284-316258-anti-gc1q-r-antibody-epr8871-western-blot.jpg "Western blot - Anti-GC1q R antibody [EPR8871] (ab131284)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8871] to GC1q R
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GC1q R antibody [EPR8871]
See all GC1q R primary antibodies -
Description
Rabbit monoclonal [EPR8871] to GC1q R -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human GC1q R aa 200-300. The exact sequence is proprietary.
Database link: Q07021 -
Positive control
- WB: HeLa, 293T, Jurkat, and Raji cell lysates. Human, mouse and rat heart tissue lysates. IHC-P: Human kidney tissue and Human tonsil tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.00 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8871 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Binds to the globular "heads" of C1Q thus inhibiting C1 activation. -
Sequence similarities
Belongs to the MAM33 family. -
Cellular localization
Mitochondrion matrix. Nucleus. Might also be nuclear in some cell types. - Information by UniProt
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Database links
- Entrez Gene: 708 Human
- Entrez Gene: 12261 Mouse
- Entrez Gene: 29681 Rat
- Omim: 601269 Human
- SwissProt: Q07021 Human
- SwissProt: O35658 Mouse
- SwissProt: O35796 Rat
- Unigene: 555866 Human
see all -
Alternative names
- ASF/SF2 associated protein p32 antibody
- C1q globular domain binding protein antibody
- C1qBP antibody
see all
Images
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Western blot - Anti-GC1q R antibody [EPR8871] (ab131284)All lanes : Anti-GC1q R antibody [EPR8871] (ab131284) at 0.01 µg/ml (purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Human heart lysates
Lane 3 : Mouse heart lysates
Lane 4 : Rat heart lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Blocking and diluting buffer: 5% NFDM/TBST -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)](https://www.abcam.com/ps/products/131/ab131284/Images/ab131284-316278-anti-gc1q-r-antibody-epr8871-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling GC1q R with Purified ab131284 at 1:250 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer (pH 6.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)](https://www.abcam.com/ps/products/131/ab131284/Images/ab131284-316277-anti-gc1q-r-antibody-epr8871-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling GC1q R with Purified ab131284 at 1:250 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer (pH 6.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)](https://www.abcam.com/ps/products/131/ab131284/Images/ab131284-316276-anti-gc1q-r-antibody-epr8871-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling GC1q R with Purified ab131284 at 1:250 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer (pH 6.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
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![Western blot - Anti-GC1q R antibody [EPR8871] (ab131284)](https://www.abcam.com/ps/products/131/ab131284/Images/ab131284-145683-anti-gc1q-r-antibody-epr8871-western-blot.jpg)
Western blot - Anti-GC1q R antibody [EPR8871] (ab131284)All lanes : Anti-GC1q R antibody [EPR8871] (ab131284) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)](https://www.abcam.com/ps/products/131/ab131284/Images/ab131284-145682-anti-gc1q-r-antibody-epr8871-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)Immunohistochemical analysis of paraffin-embedded human kidney tissue labelled with unpurified ab131284 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)](https://www.abcam.com/ps/products/131/ab131284/Images/ab131284-145681-anti-gc1q-r-antibody-epr8871-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelled with unpurified ab131284 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-GC1q R antibody [EPR8871] (ab131284)](https://www.abcam.com/ps/products/131/ab131284/Images/ab131284-145680-anti-gc1q-r-antibody-epr8871.jpg)
OI-RD Scanning - Anti-GC1q R antibody [EPR8871] (ab131284) -
![Anti-GC1q R antibody [EPR8871] (ab131284)](https://www.abcam.com/ps/products/131/ab131284/Images/ab131284-9-benefits-of-recombinant-antibodies.png)
Anti-GC1q R antibody [EPR8871] (ab131284)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab131284 has been referenced in 3 publications.
- Xie ZB et al. High cytoplasm HABP1 expression as a predictor of poor survival and late tumor stage in pancreatic ductal adenocarcinoma patients. Eur J Surg Oncol 45:207-212 (2019). PubMed: 30389300
- Jiang Y et al. Increased breast cancer risk with HABP1/p32/gC1qR genetic polymorphism rs2285747 and its upregulation in northern Chinese women. Oncotarget 8:13932-13941 (2017). PubMed: 28108744
- Kim K et al. C1QBP is upregulated in colon cancer and binds to apolipoprotein A-I. Exp Ther Med 13:2493-2500 (2017). PubMed: 28565870