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RecombinantRabMAb

Recombinant Anti-GC1q R antibody [EPR8871] (ab131284)

Submit a review Submit a question References (6)
Western blot - Anti-GC1q R antibody [EPR8871] (ab131284)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
  • Western blot - Anti-GC1q R antibody [EPR8871] (ab131284)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
  • OI-RD Scanning - Anti-GC1q R antibody [EPR8871] (ab131284)
  • Anti-GC1q R antibody [EPR8871] (ab131284)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8871] to GC1q R
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-GC1q R antibody [EPR8871]
    See all GC1q R primary antibodies

  • Description

    Rabbit monoclonal [EPR8871] to GC1q R

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GC1q R aa 200-300. The exact sequence is proprietary.
    Database link: Q07021

  • Positive control

    • WB: HeLa, 293T, Jurkat, and Raji cell lysates. Human, mouse and rat heart tissue lysates. IHC-P: Human kidney tissue and Human tonsil tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,ICC or IP.

Target

  • Function

    Binds to the globular "heads" of C1Q thus inhibiting C1 activation.

  • Sequence similarities

    Belongs to the MAM33 family.

  • Cellular localization

    Mitochondrion matrix. Nucleus. Might also be nuclear in some cell types.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ASF/SF2 associated protein p32 antibody
    • C1q globular domain binding protein antibody
    • C1qBP antibody
    • C1QBP_HUMAN antibody
    • Complement component 1 q subcomponent binding protein antibody
    • Complement component 1 Q subcomponent binding protein mitochondrial antibody
    • Complement component 1 Q subcomponent-binding protein, mitochondrial antibody
    • GC1Q R antibody
    • GC1q R protein antibody
    • GC1q-R protein antibody
    • GC1QBP antibody
    • GC1QR antibody
    • globular domain of, C1q, receptor for antibody
    • Glycoprotein gC1qBP antibody
    • HABP 1 antibody
    • HABP1 antibody
    • Hyaluronan binding protein 1 antibody
    • Hyaluronan-binding protein 1 antibody
    • Mitochondrial matrix protein p32 antibody
    • p32 antibody
    • p32 splicing factor antibody
    • p33 antibody
    • Pre mrna splicing factor SF2 P32 subunit precursor antibody
    • SF2p32 antibody
    • Splicing factor SF2 associated protein antibody
    see all

Images

  • Western blot - Anti-GC1q R antibody [EPR8871] (ab131284)
    Western blot - Anti-GC1q R antibody [EPR8871] (ab131284)
    All lanes : Anti-GC1q R antibody [EPR8871] (ab131284) at 0.01 µg/ml (purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Human heart lysates
    Lane 3 : Mouse heart lysates
    Lane 4 : Rat heart lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 31 kDa



    Blocking and diluting buffer: 5% NFDM/TBST
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling GC1q R with Purified ab131284 at 1:250 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer (pH 6.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling GC1q R with Purified ab131284 at 1:250 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer (pH 6.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling GC1q R with Purified ab131284 at 1:250 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer (pH 6.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

  • Western blot - Anti-GC1q R antibody [EPR8871] (ab131284)
    Western blot - Anti-GC1q R antibody [EPR8871] (ab131284)
    All lanes : Anti-GC1q R antibody [EPR8871] (ab131284) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 31 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labelled with unpurified ab131284 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelled with unpurified ab131284 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-GC1q R antibody [EPR8871] (ab131284)
    OI-RD Scanning - Anti-GC1q R antibody [EPR8871] (ab131284)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-GC1q R antibody [EPR8871] (ab131284)
    Anti-GC1q R antibody [EPR8871] (ab131284)

References (6)

Publishing research using ab131284? Please let us know so that we can cite the reference in this datasheet.

ab131284 has been referenced in 6 publications.

  • Sünderhauf A  et al. GC1qR Cleavage by Caspase-1 Drives Aerobic Glycolysis in Tumor Cells. Front Oncol 10:575854 (2020). PubMed: 33102234
  • Raschdorf A  et al. Heterozygous P32/C1QBP/HABP1 Polymorphism rs56014026 Reduces Mitochondrial Oxidative Phosphorylation and Is Expressed in Low-grade Colorectal Carcinomas. Front Oncol 10:631592 (2020). PubMed: 33628739
  • Liang J  et al. The role of the globular heads of the C1q receptor in TcdA-induced human colonic epithelial cell apoptosis via a mitochondria-dependent pathway. BMC Microbiol 20:274 (2020). PubMed: 32878596
  • Xie ZB  et al. High cytoplasm HABP1 expression as a predictor of poor survival and late tumor stage in pancreatic ductal adenocarcinoma patients. Eur J Surg Oncol 45:207-212 (2019). PubMed: 30389300
  • Jiang Y  et al. Increased breast cancer risk with HABP1/p32/gC1qR genetic polymorphism rs2285747 and its upregulation in northern Chinese women. Oncotarget 8:13932-13941 (2017). PubMed: 28108744
  • Kim K  et al. C1QBP is upregulated in colon cancer and binds to apolipoprotein A-I. Exp Ther Med 13:2493-2500 (2017). PubMed: 28565870

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