Recombinant
RabMAb

Recombinant Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

Overview

  • Product name

    Anti-GCAT antibody [EPR13450] - N-terminal
    See all GCAT primary antibodies
  • Description

    Rabbit monoclonal [EPR13450] to GCAT - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GCAT aa 1-150 (N terminal). The exact sequence is proprietary.
    Database link: O75600

  • Positive control

    • BxPC-3, HepG2 and U87-MG cell lysates. Human fetal liver tissue lysate. Human heart and pancreas tissue. HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC/IF 1/50 - 1/100.
IP 1/40.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificity

    Strongly expressed in heart, brain, liver and pancreas. Also found in lung.
  • Pathway

    Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 2/2.
  • Sequence similarities

    Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2 amino 3 ketobutyrate CoA ligase antibody
    • 2 amino 3 ketobutyrate coenzyme A ligase, mitochondrial antibody
    • 2-amino-3-ketobutyrate coenzyme A ligase antibody
    • AKB ligase antibody
    • Aminoacetone synthase antibody
    • GCAT antibody
    • Glycine acetyltransferase antibody
    • glycine C acetyltransferase antibody
    • glycine C acetyltransferase (2 amino 3 ketobutyrate coenzyme A ligase) antibody
    • KBL_HUMAN antibody
    • KLB antibody
    • mitochondrial antibody
    see all

Images

  • All lanes : Anti-GCAT antibody [EPR13450] - N-terminal (ab181094) at 1/5000 dilution

    Lane 1 : BxPC-3 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : U87-MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • Anti-GCAT antibody [EPR13450] - N-terminal (ab181094) at 1/5000 dilution + Human fetal liver lysate

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • Immunohistochemical analysis of Human heart tissue, staining GCAT with ab181094 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of Human pancreas tissue, staining GCAT with ab181094 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunofluorescence analysis of HepG2 cells, staining GCAT (green) with ab181094 at 1/100 dilution. An Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

  • Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate (lane 1) or negative control (lane 2), labeling GCAT immunoprecipitated using ab181094 at 1/40 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

References

ab181094 has not yet been referenced specifically in any publications.

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