Overview

  • Product name

    Anti-GCDFP 15 antibody [EP1582Y]
    See all GCDFP 15 primary antibodies
  • Description

    Rabbit monoclonal [EP1582Y] to GCDFP 15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GCDFP 15 aa 1-100 (N terminal). The exact sequence is proprietary.
    (Peptide available as ab155648)

  • Positive control

    • T47D cell lysate, human breast carcinoma tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab62363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 17 kDa.Can be blocked with GCDFP 15 peptide (ab155648).
IHC-P 1/100 - 1/250.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Tissue specificity

      Expressed in pathological conditions of the mammary gland and in several exocrine tissues, such as the lacrimal, salivary, and sweat glands.
    • Sequence similarities

      Belongs to the PIP family.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • BRST-2 antibody
      • BRST2 antibody
      • GCDFP-15 antibody
      • GCDGP15 antibody
      • gp17 antibody
      • GPIP4 antibody
      • Gross cystic disease fluid protein 15 antibody
      • PIP antibody
      • PIP_HUMAN antibody
      • Prolactin induced protein antibody
      • Prolactin inducible protein precursor antibody
      • Prolactin-induced protein antibody
      • Prolactin-inducible protein antibody
      • SABP antibody
      • Secretory actin binding protein antibody
      • Secretory actin-binding protein antibody
      see all

    Images

    • Anti-GCDFP 15 antibody [EP1582Y] (ab62363) at 1/2000 dilution + T47D cell lysate at 10 µg

      Secondary
      goat anti rabbit HRP at 1/2000 dilution

      Predicted band size: 17 kDa
      Observed band size: 17 kDa

    • Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling GCDFP 15 with ab62363 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton-X. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 Anti-Tubulin antibody (1/1000) followed by ab150120   AlexaFluor®594 Goat anti-Mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).

      For negative control 1, ab62363 at dilution of 1/100 was used followed by anti-mouse secondary antibody (ab150120) at 1/1000 dilution. For negative control 2, ab7291; 1/1000 was used followed by anti-rabbit secondary antibody (ab150077) at 1/1000 dilution.  

      Confocal image showing cytoplasmic staining on MCF7 cells.

    • Immunohistochemical analysis of GCDFP 15 expression in paraffin embedded human breast carcinoma using 1/100-1/250 dilution of ab62363
    • ab62363 showing positive staining in Cystic breast disease tissue.

    • Flow cytometry analysis of T47D (human mammary gland ductal carcinoma) (pink) with ab62363 at dilution of 1/20. The secondary antibody used was goat anti rabbit IgG (FITC) at dilution of 1/150. Cells were fixed with 2% paraformaldehyde. Isotype control antibody used was Rabbit monoclonal IgG (green).

    • ab62363 showing positive staining in Salivary gland tissue.

    • ab62363 showing negative staining in Hepatocellular carcinoma tissue.

    • ab62363 showing negative staining in Prostate hyperplasia tissue.

    References

    This product has been referenced in:

    • Guo Z  et al. Differential urinary glycoproteome analysis of type 2 diabetic nephropathy using 2D-LC-MS/MS and iTRAQ quantification. J Transl Med 13:371 (2015). WB . Read more (PubMed: 26608305) »
    • Gallo A  et al. Gross Cystic Disease Fluid Protein-15(GCDFP-15)/Prolactin-Inducible Protein (PIP) as Functional Salivary Biomarker for Primary Sjögren's Syndrome. J Genet Syndr Gene Ther 4:N/A (2013). IHC . Read more (PubMed: 24416635) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    No
    Fixative
    Methanol

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jan 28 2014

    Answer

    The antibody ab62363 has an immunogen near the N-terminus of human GCDFP 15. The peptide ab48069 you mention however is derived from the internal region of GCDFP 15 and therefore ab62363 will not bind to it.

    I have been in contact with the lab and they have agreed to add a new product to the catalogue, another GCDFP 15 peptide. This GCDFP 15 peptide is the blocking peptide of ab62363 and therefore ab62363 should bind to it since the peptide was used to raise this antibody. The catalogue number of this peptide is ab155648 and costs €‘180.

    Read More

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