Product nameAnti-GCDFP 15 antibody [EP1582Y]
See all GCDFP 15 primary antibodies
DescriptionRabbit monoclonal [EP1582Y] to GCDFP 15
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human GCDFP 15 aa 1-100 (N terminal). The exact sequence is proprietary.
(Peptide available as
- T47D cell lysate, human breast carcinoma tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab62363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Predicted molecular weight: 17 kDa.Can be blocked with GCDFP 15 peptide (ab155648).|
|IHC-P||1/100 - 1/250.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/50 - 1/100.|
Tissue specificityExpressed in pathological conditions of the mammary gland and in several exocrine tissues, such as the lacrimal, salivary, and sweat glands.
Sequence similaritiesBelongs to the PIP family.
- Information by UniProt
- BRST-2 antibody
- BRST2 antibody
- GCDFP-15 antibody
Anti-GCDFP 15 antibody [EP1582Y] (ab62363) at 1/2000 dilution + T47D cell lysate at 10 µg
goat anti rabbit HRP at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling GCDFP 15 with ab62363 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton-X. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 Anti-Tubulin antibody (1/1000) followed by ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).
For negative control 1, ab62363 at dilution of 1/100 was used followed by anti-mouse secondary antibody (ab150120) at 1/1000 dilution. For negative control 2, ab7291; 1/1000 was used followed by anti-rabbit secondary antibody (ab150077) at 1/1000 dilution.
Confocal image showing cytoplasmic staining on MCF7 cells.
Immunohistochemical analysis of GCDFP 15 expression in paraffin embedded human breast carcinoma using 1/100-1/250 dilution of ab62363
ab62363 showing positive staining in Cystic breast disease tissue.
Flow cytometry analysis of T47D (human mammary gland ductal carcinoma) (pink) with ab62363 at dilution of 1/20. The secondary antibody used was goat anti rabbit IgG (FITC) at dilution of 1/150. Cells were fixed with 2% paraformaldehyde. Isotype control antibody used was Rabbit monoclonal IgG (green).
ab62363 showing positive staining in Salivary gland tissue.
ab62363 showing negative staining in Hepatocellular carcinoma tissue.
ab62363 showing negative staining in Prostate hyperplasia tissue.
This product has been referenced in:
- Guo Z et al. Differential urinary glycoproteome analysis of type 2 diabetic nephropathy using 2D-LC-MS/MS and iTRAQ quantification. J Transl Med 13:371 (2015). WB . Read more (PubMed: 26608305) »
- Gallo A et al. Gross Cystic Disease Fluid Protein-15(GCDFP-15)/Prolactin-Inducible Protein (PIP) as Functional Salivary Biomarker for Primary Sjögren's Syndrome. J Genet Syndr Gene Ther 4:N/A (2013). IHC . Read more (PubMed: 24416635) »