Product nameAnti-GCDFP 15 antibody [EPR1582]
See all GCDFP 15 primary antibodies
DescriptionRabbit monoclonal [EPR1582] to GCDFP 15
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Human
Synthetic peptide within Human GCDFP 15. The exact sequence is proprietary.
- T47D and Human breast cancer cell lysates; Human breast carcinoma tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 6.80 x 10 -12 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Tissue specificityExpressed in pathological conditions of the mammary gland and in several exocrine tissues, such as the lacrimal, salivary, and sweat glands.
Sequence similaritiesBelongs to the PIP family.
- Information by UniProt
- BRST-2 antibody
- BRST2 antibody
- GCDFP-15 antibody
All lanes : Anti-GCDFP 15 antibody [EPR1582] (ab133290) at 1/1000 dilution
Lane 1 : T47D cell lysate
Lane 2 : Human breast cancer cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 17 kDa
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling GCDFP 15 with ab133290 at 1/100 dilution.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Li H et al. Expression and localization of Forkhead transcription factor A1 in the three-dimensional reconstructed eccrine sweat glands. Acta Histochem 120:520-524 (2018). Read more (PubMed: 29909922) »
- Zhang M et al. Time course of differentiation of different cell types in 3D-reconstructed eccrine sweat glands. J Mol Histol 49:567-575 (2018). Read more (PubMed: 30238337) »