Overview

  • Product name

    Anti-GCDFP 15 antibody [EPR1582]
    See all GCDFP 15 primary antibodies
  • Description

    Rabbit monoclonal [EPR1582] to GCDFP 15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GCDFP 15. The exact sequence is proprietary.

  • Positive control

    • T47D and Human breast cancer cell lysates; Human breast carcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Tissue specificity

      Expressed in pathological conditions of the mammary gland and in several exocrine tissues, such as the lacrimal, salivary, and sweat glands.
    • Sequence similarities

      Belongs to the PIP family.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • BRST-2 antibody
      • BRST2 antibody
      • GCDFP-15 antibody
      • GCDGP15 antibody
      • gp17 antibody
      • GPIP4 antibody
      • Gross cystic disease fluid protein 15 antibody
      • PIP antibody
      • PIP_HUMAN antibody
      • Prolactin induced protein antibody
      • Prolactin inducible protein precursor antibody
      • Prolactin-induced protein antibody
      • Prolactin-inducible protein antibody
      • SABP antibody
      • Secretory actin binding protein antibody
      • Secretory actin-binding protein antibody
      see all

    Images

    • All lanes : Anti-GCDFP 15 antibody [EPR1582] (ab133290) at 1/1000 dilution

      Lane 1 : T47D cell lysate
      Lane 2 : Human breast cancer cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 17 kDa

    • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling GCDFP 15 with ab133290 at 1/100 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Li H  et al. Expression and localization of Forkhead transcription factor A1 in the three-dimensional reconstructed eccrine sweat glands. Acta Histochem 120:520-524 (2018). Read more (PubMed: 29909922) »
    • Zhang M  et al. Time course of differentiation of different cell types in 3D-reconstructed eccrine sweat glands. J Mol Histol 49:567-575 (2018). Read more (PubMed: 30238337) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133290.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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