Anti-GCDFP 15 antibody [PIP/1571] - BSA and Azide free (ab218935)
Key features and details
- Mouse monoclonal [PIP/1571] to GCDFP 15 - BSA and Azide free
- Suitable for: Protein Array, WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-GCDFP 15 antibody [PIP/1571] - BSA and Azide free
See all GCDFP 15 primary antibodies -
Description
Mouse monoclonal [PIP/1571] to GCDFP 15 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human GCDFP 15 aa 41-146. The exact sequence is proprietary.
Database link: P12273 -
Positive control
- Human pancreas tissue lysate; HepG2 cell line lysate; Human breast carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
PIP/1571 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Protein Array |
Use at an assay dependent concentration.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 17 kDa.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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Protein Array
Use at an assay dependent concentration. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 17 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Expressed in pathological conditions of the mammary gland and in several exocrine tissues, such as the lacrimal, salivary, and sweat glands. -
Sequence similarities
Belongs to the PIP family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5304 Human
- Omim: 176720 Human
- SwissProt: P12273 Human
- Unigene: 99949 Human
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Alternative names
- BRST-2 antibody
- BRST2 antibody
- GCDFP-15 antibody
see all
Images
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This data was produced with ab218480, the same antibody in a different formulation with BSA and Azide.
ab218480 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -
All lanes : Anti-GCDFP 15 antibody [PIP/1571] - BSA and Azide free (ab218935) at 1 µg/ml
Lane 1 : Human pancreas tissue lysate
Lane 2 : HepG2 cell line lysate
Predicted band size: 17 kDa -
Immunohistochemical analysis of formalin fixed, paraffin embedded Human breast carcinoma tissue labeling GCDFP 15 with ab218935 at 1 µg/mL.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab218935 has not yet been referenced specifically in any publications.