Recombinant
RabMAb

Recombinant Anti-GCDFP 15 antibody [SP185] - BSA and Azide free (ab238776)

Overview

  • Product name

    Anti-GCDFP 15 antibody [SP185] - BSA and Azide free
    See all GCDFP 15 primary antibodies
  • Description

    Rabbit monoclonal [SP185] to GCDFP 15 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GCDFP 15 aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P12273

  • Positive control

    • IHC-P: Human breast carcinoma and breast ductal carcinoma tissues
  • General notes

    ab238776 is the carrier-free version of ab183321 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab238776 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Cow: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Tissue specificity

    Expressed in pathological conditions of the mammary gland and in several exocrine tissues, such as the lacrimal, salivary, and sweat glands.
  • Sequence similarities

    Belongs to the PIP family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • BRST-2 antibody
    • BRST2 antibody
    • GCDFP-15 antibody
    • GCDGP15 antibody
    • gp17 antibody
    • GPIP4 antibody
    • Gross cystic disease fluid protein 15 antibody
    • PIP antibody
    • PIP_HUMAN antibody
    • Prolactin induced protein antibody
    • Prolactin inducible protein precursor antibody
    • Prolactin-induced protein antibody
    • Prolactin-inducible protein antibody
    • SABP antibody
    • Secretory actin binding protein antibody
    • Secretory actin-binding protein antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling GCDFP 15 with ab183321 at 1/100 dilution (2.21 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human breast carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab183321 for 10 mins at room temperature. This image was generated using ab183321, the same clone, but with a different buffer formulation.

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling GCDFP with ab183321 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183321).

  • Immunohistochemical analysis of paraffin-embedded Human breast ductal carcinoma tissue labeling GCDFP with ab183321 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183321).

  • Immunohistochemical analysis of paraffin-embedded Human breast ductal carcinoma tissue labeling GCDFP with ab183321 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183321).

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling GCDFP with ab183321 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183321).

References

ab238776 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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