Recombinant
RabMAb

Recombinant Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)

Overview

  • Product name

    Anti-GCDFP 15 antibody [SP185] - C-terminal
    See all GCDFP 15 primary antibodies
  • Description

    Rabbit monoclonal [SP185] to GCDFP 15 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GCDFP 15 aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P12273

  • Positive control

    • WB: T47D cell lysate IHC-P: Human breast carcinoma and breast ductal carcinoma tissues
  • General notes

    Cow: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/400. Predicted molecular weight: 17 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Tissue specificity

    Expressed in pathological conditions of the mammary gland and in several exocrine tissues, such as the lacrimal, salivary, and sweat glands.
  • Sequence similarities

    Belongs to the PIP family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • BRST-2 antibody
    • BRST2 antibody
    • GCDFP-15 antibody
    • GCDGP15 antibody
    • gp17 antibody
    • GPIP4 antibody
    • Gross cystic disease fluid protein 15 antibody
    • PIP antibody
    • PIP_HUMAN antibody
    • Prolactin induced protein antibody
    • Prolactin inducible protein precursor antibody
    • Prolactin-induced protein antibody
    • Prolactin-inducible protein antibody
    • SABP antibody
    • Secretory actin binding protein antibody
    • Secretory actin-binding protein antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling GCDFP 15 with ab183321 at 1/100 dilution (2.21 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human breast carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab183321 for 10 mins at room temperature.

  • Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321) at 1/400 dilution + T47D cell lysate

    Predicted band size: 17 kDa

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling GCDFP with ab183321 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human breast ductal carcinoma tissue labeling GCDFP with ab183321 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human breast ductal carcinoma tissue labeling GCDFP with ab183321 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling GCDFP with ab183321 at 1/100 dilution.

References

ab183321 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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