Key features and details
- Rabbit polyclonal to GCDH/GCD
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-GCDH/GCD antibody
See all GCDH/GCD primary antibodies
DescriptionRabbit polyclonal to GCDH/GCD
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse
Predicted to work with: Cow, Human, Pig, Cynomolgus monkey
Recombinant fragment (His-T7-tag) corresponding to Mouse GCDH/GCD aa 270-438. N-terminal tags. Expressed in E. coli.
EVPEENVLPNVSSLAGPFGCLNTARYGITWGVLGAAEFCLHTARQYALDR IQFGVPLARNQLVQKKLADMLTEITLGLHACLQLGRLKDQDKATPEMVSM LKRNNCGKALDIARQARDILGGNGISDEYHVIRHAMNLEAVNTYEGTHDI HALILGRAITGIQAFTVGK
Database link: Q60759
- IHC-P: Mouse liver tissue. WB: Recombinant mouse GCDH/GCD protein. Mouse liver and heart tissue lysate.
This product was previously labelled as GCDH
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography
Our Abpromise guarantee covers the use of ab231783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionCatalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. Isoform Short is inactive.
Tissue specificityIsoform 1 and isoform 2 are expressed in fibroblasts and liver.
PathwayAmino-acid metabolism; lysine degradation.
Amino-acid metabolism; tryptophan metabolism.
Involvement in diseaseDefects in GCDH are the cause of glutaric aciduria type 1 (GA1) [MIM:231670]. GA1 is an autosomal recessive metabolic disorder characterized by progressive dystonia and athetosis due to gliosis and neuronal loss in the basal ganglia.
Sequence similaritiesBelongs to the acyl-CoA dehydrogenase family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- ACAD5 antibody
- EC 220.127.116.11 antibody
- GCD antibody
Formalin-fixed, paraffin-embedded mouse liver tissue stained for GCDH/GCD with ab231783 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-GCDH/GCD antibody (ab231783) at 1 µg/ml + Mouse liver tissue lysate
Anti-GCDH/GCD antibody (ab231783) at 1 µg/ml + Mouse heart tissue lysate
Anti-GCDH/GCD antibody (ab231783) at 2 µg/ml + Recombinant mouse GCDH/GCD protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231783 has not yet been referenced specifically in any publications.