Overview

  • Product name

  • Description

    Mouse polyclonal to GCDH/GCD
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human GCDH/GCD.

  • Positive control

    • Human liver tissue lysate and 293T cell lysate
  • General notes

     This product was previously labelled as GCDH

     

Properties

Applications

Our Abpromise guarantee covers the use of ab67103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects bands of approximately 46 and 48 kDa (predicted molecular weight: 48 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. Isoform Short is inactive.
    • Tissue specificity

      Isoform 1 and isoform 2 are expressed in fibroblasts and liver.
    • Pathway

      Amino-acid metabolism; lysine degradation.
      Amino-acid metabolism; tryptophan metabolism.
    • Involvement in disease

      Defects in GCDH are the cause of glutaric aciduria type 1 (GA1) [MIM:231670]. GA1 is an autosomal recessive metabolic disorder characterized by progressive dystonia and athetosis due to gliosis and neuronal loss in the basal ganglia.
    • Sequence similarities

      Belongs to the acyl-CoA dehydrogenase family.
    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • ACAD5 antibody
      • EC 1.3.99.7 antibody
      • GCD antibody
      • Gcdh antibody
      • GCDH_HUMAN antibody
      • Glutaryl CoA dehydrogenase antibody
      • Glutaryl CoA dehydrogenase, mitochondrial antibody
      • Glutaryl Coenzyme A dehydrogenase antibody
      • Glutaryl-CoA dehydrogenase antibody
      • mitochondrial antibody
      • MS781 antibody
      see all

    Images

    • Anti-GCDH/GCD antibody (ab67103) at 1/500 dilution + human liver tissue lysate at 25 µg/ml

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa

    • All lanes : Anti-GCDH/GCD antibody (ab67103) at 1/500 dilution

      Lane 1 : GCDH/GCD transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa
      Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.

    References

    ab67103 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab67103.
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