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Synthetic peptide within Human GCET1 aa 201-230 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q86WD7
Our Abpromise guarantee covers the use of ab176176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 47 kDa.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lymph tissue labeling GCET1 with ab176176 at 1/10 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of MDA-231 cells labelling GCET1 using ab176176 (bottom histogram) or negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab176176 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"