Overview

  • Product name

  • Description

    Rabbit polyclonal to GCET2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GCET2 aa 128-178. The exact sequence is proprietary.
    Database link: Q8N6F7

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to GCET2 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab242050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 21 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation.
    • Tissue specificity

      Expressed in diffuse large B-cell lymphoma (DLBCL) and several germinal center (GC)-like lymphoma cell lines (at protein level). Highly expressed in normal GC lymphocytes and GC-derived malignancies. Expressed in thymus and spleen.
    • Post-translational
      modifications

      Phosphorylation on tyrosine residues can be induced by IL6. Phosphorylation is mediated by LYN.
    • Cellular localization

      Cytoplasm. Cell membrane. It relocalizes from the cytoplasm to podosome-like structures upon cell treatment with IL6.
    • Information by UniProt
    • Database links

    • Alternative names

      • GCAT2 antibody
      • Gcet antibody
      • GCET2 germinal center expressed transcript 2 antibody
      • GCET2 germinal centre expressed transcript 2 antibody
      • Gcsam antibody
      • GCSAM_HUMAN antibody
      • Germinal center associated lymphoma antibody
      • Germinal center associated lymphoma protein antibody
      • Germinal center B cell associated protein 2 antibody
      • Germinal center B-cell-expressed transcript 2 protein antibody
      • Germinal center expressed transcript 2 antibody
      • Germinal center-associated lymphoma protein antibody
      • Germinal center-associated signaling and motility protein antibody
      • Germinal centre associated lymphoma antibody
      • Germinal centre associated lymphoma protein antibody
      • Germinal centre B cell associated protein 2 antibody
      • Germinal centre expressed transcript 2 antibody
      • hGAL antibody
      • M17 antibody
      • M17 L antibody
      see all

    Images

    • All lanes : Anti-GCET2 antibody (ab242050) at 0.1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 21 kDa


      Exposure time: 30 seconds


      Lysates prepared in NETN buffer.

    References

    ab242050 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab242050.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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