Recombinant
RabMAb

Recombinant Anti-GCET2 antibody [EPR14333-60] (ab185539)

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Overview

  • Product name

    Anti-GCET2 antibody [EPR14333-60]
    See all GCET2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14333-60] to GCET2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein within Human GCET2 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8N6F7

  • Positive control

    • Raji and Jurkat cell lysates. Jurkat cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14333-60

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab185539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
Flow Cyt 1/130.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation.

  • Tissue specificity

    Expressed in diffuse large B-cell lymphoma (DLBCL) and several germinal center (GC)-like lymphoma cell lines (at protein level). Highly expressed in normal GC lymphocytes and GC-derived malignancies. Expressed in thymus and spleen.

  • Post-translational
    modifications

    Phosphorylation on tyrosine residues can be induced by IL6. Phosphorylation is mediated by LYN.

  • Cellular localization

    Cytoplasm. Cell membrane. It relocalizes from the cytoplasm to podosome-like structures upon cell treatment with IL6.
  • Information by UniProt

  • Database links

    • Alternative names

      • GCAT2 antibody
      • Gcet antibody
      • GCET2 germinal center expressed transcript 2 antibody
      • GCET2 germinal centre expressed transcript 2 antibody
      • Gcsam antibody
      • GCSAM_HUMAN antibody
      • Germinal center associated lymphoma antibody
      • Germinal center associated lymphoma protein antibody
      • Germinal center B cell associated protein 2 antibody
      • Germinal center B-cell-expressed transcript 2 protein antibody
      • Germinal center expressed transcript 2 antibody
      • Germinal center-associated lymphoma protein antibody
      • Germinal center-associated signaling and motility protein antibody
      • Germinal centre associated lymphoma antibody
      • Germinal centre associated lymphoma protein antibody
      • Germinal centre B cell associated protein 2 antibody
      • Germinal centre expressed transcript 2 antibody
      • hGAL antibody
      • M17 antibody
      • M17 L antibody
      see all

    Images

    • Flow Cytometry - Anti-GCET2 antibody [EPR14333-60] (ab185539)
      Flow Cytometry - Anti-GCET2 antibody [EPR14333-60] (ab185539)

      Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat cells labeling GCET2 with ab185539 at 1/130 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

    • Western blot - Anti-GCET2 antibody [EPR14333-60] (ab185539)
      Western blot - Anti-GCET2 antibody [EPR14333-60] (ab185539)
      All lanes : Anti-GCET2 antibody [EPR14333-60] (ab185539) at 1/5000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Jukat cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 21 kDa
      Observed band size: 21 kDa

    References

    ab185539 has not yet been referenced specifically in any publications.

    Publishing research using ab185539? Please let us know so that we can cite the reference in this datasheet.

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