Recombinant Anti-GCET2 antibody [EPR14333-60] (ab185539)
![Flow Cytometry - Anti-GCET2 antibody [EPR14333-60] (ab185539)](https://www.abcam.com/ps/products/185/ab185539/Images/ab185539-219792-ab1855392.jpg "Flow Cytometry - Anti-GCET2 antibody [EPR14333-60] (ab185539)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14333-60] to GCET2
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-GCET2 antibody [EPR14333-60]
See all GCET2 primary antibodies -
Description
Rabbit monoclonal [EPR14333-60] to GCET2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human GCET2 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q8N6F7 -
Positive control
- Raji and Jurkat cell lysates. Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14333-60 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab185539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). | |
| Flow Cyt | 1/130. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation. -
Tissue specificity
Expressed in diffuse large B-cell lymphoma (DLBCL) and several germinal center (GC)-like lymphoma cell lines (at protein level). Highly expressed in normal GC lymphocytes and GC-derived malignancies. Expressed in thymus and spleen. -
Post-translational
modificationsPhosphorylation on tyrosine residues can be induced by IL6. Phosphorylation is mediated by LYN. -
Cellular localization
Cytoplasm. Cell membrane. It relocalizes from the cytoplasm to podosome-like structures upon cell treatment with IL6. - Information by UniProt
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Database links
- Entrez Gene: 257144 Human
- Omim: 607792 Human
- SwissProt: Q8N6F7 Human
- Unigene: 49614 Human
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Alternative names
- GCAT2 antibody
- Gcet antibody
- GCET2 germinal center expressed transcript 2 antibody
see all
Images
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Flow Cytometry - Anti-GCET2 antibody [EPR14333-60] (ab185539)
Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat cells labeling GCET2 with ab185539 at 1/130 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
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![Western blot - Anti-GCET2 antibody [EPR14333-60] (ab185539)](https://www.abcam.com/ps/products/185/ab185539/Images/ab185539-219791-ab185539.jpg)
Western blot - Anti-GCET2 antibody [EPR14333-60] (ab185539)All lanes : Anti-GCET2 antibody [EPR14333-60] (ab185539) at 1/5000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Jukat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
![Anti-GCET2 antibody [EPR14333-60] (ab185539)](https://www.abcam.com/ps/products/185/ab185539/Images/ab185539-3-benefits-of-recombinant-antibodies.png)
Anti-GCET2 antibody [EPR14333-60] (ab185539)
Datasheets and documents
Certificate of Compliance
References (0)
ab185539 has not yet been referenced specifically in any publications.
