Recombinant
RabMAb

Recombinant Anti-GCET2 antibody [EPR14333] (ab185231)

Overview

  • Product name

    Anti-GCET2 antibody [EPR14333]
    See all GCET2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14333] to GCET2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human GCET2 aa 1 to the C-terminus.
    Database link: Q8N6F7

  • Positive control

    • Human Non Hodgkin's lymphoma and Human diffuse large B-cell lymphoma tissues; Raji cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14333
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab185231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000.
Flow Cyt 1/230.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/500.

Target

  • Function

    Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation.
  • Tissue specificity

    Expressed in diffuse large B-cell lymphoma (DLBCL) and several germinal center (GC)-like lymphoma cell lines (at protein level). Highly expressed in normal GC lymphocytes and GC-derived malignancies. Expressed in thymus and spleen.
  • Post-translational
    modifications

    Phosphorylation on tyrosine residues can be induced by IL6. Phosphorylation is mediated by LYN.
  • Cellular localization

    Cytoplasm. Cell membrane. It relocalizes from the cytoplasm to podosome-like structures upon cell treatment with IL6.
  • Information by UniProt
  • Database links

  • Alternative names

    • GCAT2 antibody
    • Gcet antibody
    • GCET2 germinal center expressed transcript 2 antibody
    • GCET2 germinal centre expressed transcript 2 antibody
    • Gcsam antibody
    • GCSAM_HUMAN antibody
    • Germinal center associated lymphoma antibody
    • Germinal center associated lymphoma protein antibody
    • Germinal center B cell associated protein 2 antibody
    • Germinal center B-cell-expressed transcript 2 protein antibody
    • Germinal center expressed transcript 2 antibody
    • Germinal center-associated lymphoma protein antibody
    • Germinal center-associated signaling and motility protein antibody
    • Germinal centre associated lymphoma antibody
    • Germinal centre associated lymphoma protein antibody
    • Germinal centre B cell associated protein 2 antibody
    • Germinal centre expressed transcript 2 antibody
    • hGAL antibody
    • M17 antibody
    • M17 L antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human diffuse large B-cell lymphoma tissue labeling GCET2 with ab185231 at 1/2000.

  • Flow Cytometrical analysis of Raji cells labeling GCET2 with ab185231 at 1/230 and Goat anti rabbit IgG (FITC) at 1/150 as a secondary antibody compared to a negative control antibody.

  • Immunofluorescent analysis of Raji cells labeling GCET2 with ab185231 at 1/500 (red) and DAPI staining (blue). Cells fixed in -20℃ Acetone.

  • Immunohistochemical analysis of paraffin embedded Human Non Hodgkin's lymphoma tissue labeling GCET2 with ab185231 at 1/2000.

References

ab185231 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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