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  1. Link

    gch1-antibody-oti5a1-ab236387.pdf

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Neuroscience Neurotransmitter Biogenic Amines Dopamine
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Anti-GCH1 antibody [OTI5A1] (ab236387)

  • Datasheet
  • SDS
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Western blot - Anti-GCH1 antibody [OTI5A1] (ab236387)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (ab236387)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (ab236387)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (ab236387)

Key features and details

  • Mouse monoclonal [OTI5A1] to GCH1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-GCH1 antibody [OTI5A1]
    See all GCH1 primary antibodies
  • Description

    Mouse monoclonal [OTI5A1] to GCH1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant full length protein corresponding to Human GCH1 aa 1 to the C-terminus. Produced in E.coli (NP_001019195).
    Database link: P30793-1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HEK-293T cell lysate transfected with pCMV6-ENTRY GCH1 cDNA for 48 hrs. IHC-P: Human skin, placenta and spleen carcinoma tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from cell culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    OTI5A1
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Dopamine
    • Neuroscience
    • Neurotransmission
    • Nitric Oxide
    • NOS
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • GTP
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Neuroscience
    • Development

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab236387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000. Predicted molecular weight: 28 kDa.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/2000. Predicted molecular weight: 28 kDa.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Positively regulates nitric oxide synthesis in umbilical vein endothelial cells (HUVECs). May be involved in dopamine synthesis. May modify pain sensitivity and persistence. Isoform GCH-1 is the functional enzyme, the potential function of the enzymatically inactive isoforms remains unknown.
  • Tissue specificity

    In epidermis, expressed predominantly in basal undifferentiated keratinocytes and in some but not all melanocytes (at protein level).
  • Pathway

    Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.
  • Involvement in disease

    Defects in GCH1 are the cause of GTP cyclohydrolase 1 deficiency (GCH1D) [MIM:233910]; also known as atypical severe phenylketonuria due to GTP cyclohydrolase I deficiency;. GCH1D is one of the causes of malignant hyperphenylalaninemia due to tetrahydrobiopterin deficiency. It is also responsible for defective neurotransmission due to depletion of the neurotransmitters dopamine and serotonin. The principal symptoms include: psychomotor retardation, tonicity disorders, convulsions, drowsiness, irritability, abnormal movements, hyperthermia, hypersalivation, and difficulty swallowing. Some patients may present a phenotype of intermediate severity between severe hyperphenylalaninemia and mild dystonia type 5 (dystonia-parkinsonism with diurnal fluctuation). In this intermediate phenotype, there is marked motor delay, but no mental retardation and only minimal, if any, hyperphenylalaninemia.
    Defects in GCH1 are the cause of dystonia type 5 (DYT5) [MIM:128230]; also known as progressive dystonia with diurnal fluctuation, autosomal dominant Segawa syndrome or dystonia-parkinsonism with diurnal fluctuation. DYT5 is a DOPA-responsive dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT5 typically presents in childhood with walking problems due to dystonia of the lower limbs and worsening of the dystonia towards the evening. It is characterized by postural and motor disturbances showing marked diurnal fluctuation. Torsion of the trunk is unusual. Symptoms are alleviated after sleep and aggravated by fatigue and excercise. There is a favorable response to L-DOPA without side effects.
  • Sequence similarities

    Belongs to the GTP cyclohydrolase I family.
  • Post-translational
    modifications

    Phosphorylated by casein kinase II at Ser-81 in HAECs during oscillatory shear stress; phosphorylation at Ser-81 results in increased enzyme activity.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession P30793 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2643 Human
    • Entrez Gene: 29244 Rat
    • Omim: 600225 Human
    • SwissProt: P30793 Human
    • SwissProt: P22288 Rat
    • Unigene: 624900 Human
    • Unigene: 86724 Human
    • Unigene: 28195 Rat
    • Alternative names

      • dystonia 14 antibody
      • DYT 5 antibody
      • DYT14 antibody
      • DYT5 antibody
      • DYT5a antibody
      • GCH 1 antibody
      • GCH antibody
      • Gch1 antibody
      • GCH1_HUMAN antibody
      • GTP CH 1 antibody
      • GTP CH I antibody
      • GTP cyclohydrolase 1 (dopa responsive dystonia) antibody
      • GTP cyclohydrolase 1 antibody
      • GTP cyclohydrolase I antibody
      • GTP-CH-I antibody
      • GTPCH 1 antibody
      • GTPCH1 antibody
      • Guanosine 5' triphosphate cyclohydrolase I antibody
      • HPABH4B antibody
      see all

    Images

    • Western blot - Anti-GCH1 antibody [OTI5A1] (ab236387)
      Western blot - Anti-GCH1 antibody [OTI5A1] (ab236387)
      All lanes : Anti-GCH1 antibody [OTI5A1] (ab236387) at 1/2000 dilution

      Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs
      Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY GCH1 cDNA for 48 hrs

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 28 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (ab236387)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (ab236387)

      Paraffin-embedded human skin tissue stained for GCH1 using ab236387 at 1/500 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (ab236387)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (ab236387)

      Paraffin-embedded human placenta tissue stained for GCH1 using ab236387 at 1/500 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (ab236387)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [OTI5A1] (ab236387)

      Paraffin-embedded human spleen carcinoma tissue stained for GCH1 using ab236387 at 1/500 dilution in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab236387? Please let us know so that we can cite the reference in this datasheet.

    ab236387 has been referenced in 2 publications.

    • Tang Z  et al. Exendin-4 reverses endothelial dysfunction in mice fed a high-cholesterol diet by a GTP cyclohydrolase-1/tetrahydrobiopterin pathway. Mol Med Rep 18:3350-3358 (2018). PubMed: 30085331
    • Pickert G  et al. Inhibition of GTP cyclohydrolase attenuates tumor growth by reducing angiogenesis and M2-like polarization of tumor associated macrophages. Int J Cancer 132:591-604 (2013). PubMed: 22753274

    Customer reviews and Q&As

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