Anti-GCIP interacting protein p29 antibody [OTI6A5] (ab236417)
Key features and details
- Mouse monoclonal [OTI6A5] to GCIP interacting protein p29
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-GCIP interacting protein p29 antibody [OTI6A5] -
Description
Mouse monoclonal [OTI6A5] to GCIP interacting protein p29 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human GCIP interacting protein p29 aa 1-100. Produced in E.coli (NP_056299).
Database link: O95926 -
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY GCIP interacting protein p29 cDNA. IHC-P: Human colon, ovary, pancreas, pancreas carcinoma, thyroid, prostate, bladder carcinoma, lymph node, lymphoma and tonsil tissues.
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General notes
Clone OTI6A5 (formerly 6A5)
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI6A5 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/150.
Perform heat-induced epitope retrieval by Tris-EDTA, pH 8.0. |
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WB |
1/2000. Predicted molecular weight: 29 kDa.
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Notes |
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IHC-P
1/150. Perform heat-induced epitope retrieval by Tris-EDTA, pH 8.0. |
WB
1/2000. Predicted molecular weight: 29 kDa. |
Target
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Function
May be involved in pre-mRNA splicing. -
Tissue specificity
Abundantly expressed in the heart, skeletal muscle and kidney. Expressed at lower level other tissues. -
Sequence similarities
Belongs to the SYF2 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 25949 Human
- Omim: 607090 Human
- SwissProt: O95926 Human
- Unigene: 20013 Human
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Alternative names
- CBPIN antibody
- CCNDBP1 interactor antibody
- CCNDBP1-interactor antibody
see all
Images
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All lanes : Anti-GCIP interacting protein p29 antibody [OTI6A5] (ab236417) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY GCIP interacting protein p29 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 29 kDa -
Paraffin-embedded human colon tissue stained for GCIP interacting protein p29 using ab236417 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human ovary tissue stained for GCIP interacting protein p29 using ab236417 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human pancreas tissue stained for GCIP interacting protein p29 using ab236417 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human pancreas carcinoma tissue stained for GCIP interacting protein p29 using ab236417 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human thyroid tissue stained for GCIP interacting protein p29 using ab236417 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human prostate tissue stained for GCIP interacting protein p29 using ab236417 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human bladder carcinoma tissue stained for GCIP interacting protein p29 using ab236417 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human lymph node tissue stained for GCIP interacting protein p29 using ab236417 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human lymphoma tissue stained for GCIP interacting protein p29 using ab236417 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human tonsil tissue stained for GCIP interacting protein p29 using ab236417 at 1/150 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab236417 has been referenced in 1 publication.
- Liu B et al. Influence of miR-376c-3p/SYF2 Axis on the Progression of Gastric Cancer. Technol Cancer Res Treat 18:1533033819874808 (2019). PubMed: 31522605