Anti-GCLC antibody (ab41463)

Rabbit polyclonal GCLC antibody. Validated in WB and tested in Mouse, Rat, Human. Cited in 16 publication(s). Immunogen corresponding to synthetic peptide.

Overview

  • Product name

  • Description

    Rabbit polyclonal to GCLC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human GCLC aa 50-150 conjugated to keyhole limpet haemocyanin.

  • Positive control

    • This antibody gave a positive signal in the following Human Tissue Lysates: Liver, Kidney and Lung (Adenocarcinoma). This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab41463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).

Target

Images

  • All lanes : Anti-GCLC antibody (ab41463) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Human lung tissue lysate (adenocarcinoma) - insoluble fraction (female, 80 years) (ab42923)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 73 kDa
    Observed band size: 73 kDa
    Additional bands at: 58 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

  • Song CH  et al. Effects of 17ß-estradiol on colorectal cancer development after azoxymethane/dextran sulfate sodium treatment of ovariectomized mice. Biochem Pharmacol 164:139-151 (2019). Read more (PubMed: 30981879) »
  • Cho SS  et al. Cudrania Tricuspidata Extract and Its Major Constituents Inhibit Oxidative Stress-Induced Liver Injury. J Med Food 22:602-613 (2019). Read more (PubMed: 31045470) »
See all 19 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.
Unfortunately, the antibody was discontinued by the supplying lab.
The immunogens are derived from very different positions (ab17926 from AA295-313 of rat, ab41463 within residues 50 - 150 of Human GCLC). However, the immunogen of ab41463 shows a 100% similarity to a sequence found in rat.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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