Anti-GCLC antibody (ab53179)
Key features and details
- Rabbit polyclonal to GCLC
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GCLC antibody
See all GCLC primary antibodies -
Description
Rabbit polyclonal to GCLC -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Rat GCLC aa 250-350 (internal sequence). The exact sequence is proprietary.
Database link: P48506 -
Positive control
- IHC-P: Human testis tissue. WB: Kidney lysate
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB | (1) |
Use at an assay dependent concentration.
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Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. |
Target
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Pathway
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. -
Involvement in disease
Defects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450]. -
Sequence similarities
Belongs to the glutamate--cysteine ligase type 3 family. - Information by UniProt
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Database links
- Entrez Gene: 2729 Human
- Entrez Gene: 14629 Mouse
- Entrez Gene: 25283 Rat
- Omim: 606857 Human
- SwissProt: P48506 Human
- SwissProt: P97494 Mouse
- SwissProt: P19468 Rat
- Unigene: 654465 Human
see all -
Alternative names
- EC 6.3.2.2 antibody
- Gamma ECS antibody
- Gamma glutamylcysteine synthetase antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (34)
ab53179 has been referenced in 34 publications.
- Lohakul J et al. Mitochondria-Targeted Hydrogen Sulfide Delivery Molecules Protect Against UVA-Induced Photoaging in Human Dermal Fibroblasts, and in Mouse Skin In Vivo. Antioxid Redox Signal 36:1268-1288 (2022). PubMed: 34235951
- Ma R et al. Antitumor effects of Andrographis via ferroptosis-associated genes in gastric cancer. Oncol Lett 22:523 (2021). PubMed: 34025790
- Wu DM et al. Nrf2 overexpression rescues the RPE in mouse models of retinitis pigmentosa. JCI Insight 6:N/A (2021). PubMed: 33491671
- Esparza-Moltó PB et al. Generation of mitochondrial reactive oxygen species is controlled by ATPase inhibitory factor 1 and regulates cognition. PLoS Biol 19:e3001252 (2021). PubMed: 33983919
- Shimura T et al. Enhanced anti-cancer activity of andrographis with oligomeric proanthocyanidins through activation of metabolic and ferroptosis pathways in colorectal cancer. Sci Rep 11:7548 (2021). PubMed: 33824419