Recombinant
RabMAb

Recombinant Anti-GCLC antibody [EPR13476(B)] (ab181839)

Overview

  • Product name

    Anti-GCLC antibody [EPR13476(B)]
    See all GCLC primary antibodies
  • Description

    Rabbit monoclonal [EPR13476(B)] to GCLC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GCLC aa 600 to the C-terminus. The exact sequence is proprietary.
    Database link: P48506

  • Positive control

    • HepG2, A549 and Human fetal liver lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).

Target

  • Pathway

    Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
  • Involvement in disease

    Defects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450].
  • Sequence similarities

    Belongs to the glutamate--cysteine ligase type 3 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 6.3.2.2 antibody
    • Gamma ECS antibody
    • Gamma glutamylcysteine synthetase antibody
    • Gamma-ECS antibody
    • Gamma-glutamylcysteine synthetase antibody
    • GCL antibody
    • Gclc antibody
    • GCS antibody
    • GCS heavy chain antibody
    • GLCL antibody
    • GLCLC antibody
    • Glutamate cysteine ligase catalytic subunit antibody
    • Glutamate--cysteine ligase catalytic subunit antibody
    • GSH1_HUMAN antibody
    see all

Images

  • Anti-GCLC antibody [EPR13476(B)] (ab181839) at 1/5000 dilution + HepG2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 73 kDa
    Observed band size: 73 kDa

  • All lanes : Anti-GCLC antibody [EPR13476(B)] (ab181839) at 1/10000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 73 kDa
    Observed band size: 73 kDa

References

ab181839 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Lens, Spleen, Lung, Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Lens, Spleen, Lung, Kidney
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Xingjun Fan

Verified customer

Submitted Apr 18 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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