Key features and details
- Rabbit polyclonal to GCLM
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GCLM antibody
See all GCLM primary antibodies
DescriptionRabbit polyclonal to GCLM
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Synthetic peptide, corresponding to a region within amino acids 29-120 of Human GCLM (Uniprot ID: P48507).
- H1299, 293T, A431, HeLa, HepG2, MOLT4 and Raji whole cell lysates; Human BT474 xenograft tissue; HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab154017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 31 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
Tissue specificityIn all tissues examined. Highest levels in skeletal muscle.
PathwaySulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
Sequence similaritiesBelongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.
- Information by UniProt
- Gamma ECS regulatory subunit antibody
- Gamma-ECS regulatory subunit antibody
- Gamma-glutamylcysteine synthetase regulatory subunit antibody
Anti-GCLM antibody (ab154017) at 1/5000 dilution + HT1299 whole cell lysate at 30 µg
Predicted band size: 31 kDa
12% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human BT474 xenograft tissue labeling GCML with ab154017 at 1/500 dilution.
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling GCLM with ab154017 at 1/200 dilution. Lower image is merged with DNA probe.
ab154017 has not yet been referenced specifically in any publications.