Overview

  • Product name

  • Description

    Rabbit polyclonal to GCLM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 29-120 of Human GCLM (Uniprot ID: P48507).

  • Positive control

    • H1299, 293T, A431, HeLa, HepG2, MOLT4 and Raji whole cell lysates; Human BT474 xenograft tissue; HeLa cells.

Applications

Our Abpromise guarantee covers the use of ab154017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-GCLM antibody (ab154017) at 1/5000 dilution + HT1299 whole cell lysate at 30 µg

    Predicted band size: 31 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human BT474 xenograft tissue labeling GCML with ab154017 at 1/500 dilution.
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling GCLM with ab154017 at 1/200 dilution. Lower image is merged with DNA probe.

References

ab154017 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154017.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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