Overview

  • Product name

  • Description

    Rabbit polyclonal to GCLM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A synthetic peptide corresponding to a region within internal amino acids 180-229 QVKPNSNQVN LASCCVMPPD LTAFAKQFDI QLLTHNDPKE LLSEASFQEA of Human GCLM.

  • Positive control

    • MCF7 Cell Lysate

Applications

Our Abpromise guarantee covers the use of ab81445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Western blot analysis of 40µg Mouse heart lysate (lanes 1 and 2) labeling GCLM with ab81445 at 1:1000. A HRP-conjugated anti-rabbit (1:10000) was used as the secondary antibody.
  • Anti-GCLM antibody (ab81445) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 31 kDa
    Observed band size: 31 kDa



    Gel Concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling GCLM with ab81445 at 2.5 µg/ml.

References

This product has been referenced in:

  • McGann JC & Mandel G Neuronal activity induces glutathione metabolism gene expression in astrocytes. Glia N/A:N/A (2018). Read more (PubMed: 30043519) »
  • Ma YF  et al. Nuclear factor erythroid 2-related factor 2 antioxidant response element pathways protect bovine mammary epithelial cells against H2O2-induced oxidative damage in vitro. J Dairy Sci 101:5329-5344 (2018). WB . Read more (PubMed: 29573798) »
See all 9 Publications for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart (young & old mouse))
Loading amount
30 µg
Specification
Heart (young & old mouse)
Gel Running Conditions
Non-reduced Denaturing (10-12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C

Mr. Mtuhtu S.Gounder

Verified customer

Submitted Nov 15 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up