Anti-GCLM antibody [EPR6667] (ab126704)
- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-GCLM antibody [EPR6667]
See all GCLM primary antibodies -
DescriptionRabbit monoclonal [EPR6667] to GCLM
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cytmore details
Unsuitable for: ICC -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human GCLM aa 50-150. The exact sequence is proprietary.
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Positive control
- HeLa, A673, PC12, A431, and K562 cell lysates; Human breast carcinoma tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR6667
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab126704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 31 kDa. | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Tissue specificityIn all tissues examined. Highest levels in skeletal muscle.
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PathwaySulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
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Sequence similaritiesBelongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.
- Information by UniProt
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Database links
- Entrez Gene: 2730 Human
- Entrez Gene: 14630 Mouse
- Entrez Gene: 29739 Rat
- Omim: 601176 Human
- SwissProt: P48507 Human
- SwissProt: O09172 Mouse
- SwissProt: P48508 Rat
- Unigene: 315562 Human
see all -
Alternative names
- Gamma ECS regulatory subunit antibody
- Gamma-ECS regulatory subunit antibody
- Gamma-glutamylcysteine synthetase regulatory subunit antibody
see all
Images
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![Western blot - Anti-GCLM antibody [EPR6667] (ab126704)](https://www.abcam.com/ps/products/126/ab126704/Images/ab126704-313678-Ab126704KOWBJRIZ.jpg)
Western blot - Anti-GCLM antibody [EPR6667] (ab126704)All lanes : Anti-GCLM antibody [EPR6667] (ab126704) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : GCLM knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 31 kDaLanes 1 - 3: Merged signal (red and green). Green - ab126704 observed at 31 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab126704 was shown to recognize GCLM in wild-type HAP1 cells as signal was lost at the expected MW in GCLM knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GCLM knockout samples were subjected to SDS-PAGE. Ab126704 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. -
![Flow Cytometry - Anti-GCLM antibody [EPR6667] (ab126704)](https://www.abcam.com/ps/products/126/ab126704/Images/ab126704-3-ab126704FC.jpg)
Flow Cytometry - Anti-GCLM antibody [EPR6667] (ab126704)Overlay histogram showing HeLa cells stained with ab126704 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126704, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667] (ab126704)](https://www.abcam.com/ps/products/126/ab126704/Images/GCLM-Primary-antibodies-ab126704-4.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6667] (ab126704)ab126704, at 1/50 dilution, staining GCLM in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. -
![Western blot - Anti-GCLM antibody [EPR6667] (ab126704)](https://www.abcam.com/ps/products/126/ab126704/Images/GCLM-Primary-antibodies-ab126704-3.jpg)
Western blot - Anti-GCLM antibody [EPR6667] (ab126704)All lanes : Anti-GCLM antibody [EPR6667] (ab126704) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A673 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : A431 cell lysate
Lane 5 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 31 kDa
Protocols
References
This product has been referenced in:
- Sun W et al. Proanthocyanidins Attenuation of H2O2-Induced Oxidative Damage in Tendon-Derived Stem Cells via Upregulating Nrf-2 Signaling Pathway. Biomed Res Int 2017:7529104 (2017). Read more (PubMed: 29201913) »
- Zhang J et al. Comparisons of ethanol extracts of chinese propolis (poplar type) and poplar gums based on the antioxidant activities and molecular mechanism. Evid Based Complement Alternat Med 2015:307594 (2015). Mouse . Read more (PubMed: 25802536) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"