Western blot abreview for Anti-GCN2 antibody [EPR5970(2)]

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (primary chondrocytes)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30000 cells
Specification
primary chondrocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS/T
Dilution
1/500

Secondary antibody

Dilution
1/3000
Name
Non-Abcam antibody was used: Polyclonal Swine Anti-Rabbit Immunglobulins/HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 220 kDa Non-specific: 90, 50 kDa
Positive control
HEK whole cell lysate

Veronika Georgieva

Verified customer

Submitted Nov 26 2018

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