Product nameAnti-GCN2 (phospho T667) antibody [EPR2319Y]
See all GCN2 primary antibodies
DescriptionRabbit monoclonal [EPR2319Y] to GCN2 (phospho T667)
SpecificityDetects GCN2 only when phosphorylyated at threonine 667.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Synthetic phosphopeptide corresponding to residues surrounding threonine 667 of human GCN2.
- HeLa cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab68427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 220 kDa (predicted molecular weight: 220 kDa).|
FunctionCan phosphorylate the alpha subunit of EIF2 and may mediate translational control.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 protein kinase domains.
Contains 1 RWD domain.
DomainKinase domain 1 is a degenerate kinase domain.
RWD domain is reported to interact with GCN1L1.
modificationsAutophosphorylated on threonine residues.
- Information by UniProt
- E2AK4_HUMAN antibody
- Eif2ak4 antibody
- Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody
All lanes : Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427) at 1/2000 dilution
Lane 1 : HeLa cell lysate - untreated
Lane 2 : HeLa cell lysate - treated with LP
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution
Predicted band size: 220 kDa
Observed band size: 220 kDa
Additional bands at: 180 kDa. We are unsure as to the identity of these extra bands.
ab68427 has not yet been referenced specifically in any publications.