Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2320Y] to GCN2 (phospho T899) - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Human
Product nameAnti-GCN2 (phospho T899) antibody [EPR2320Y] - BSA and Azide free
See all GCN2 primary antibodies
DescriptionRabbit monoclonal [EPR2320Y] to GCN2 (phospho T899) - BSA and Azide free
Tested applicationsSuitable for: Dot blot, WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human GCN2 (phospho T899). The exact sequence is proprietary.
ab157776 is the carrier-free version of ab75836 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab157776 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab157776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 220 kDa (predicted molecular weight: 187 kDa).|
FunctionCan phosphorylate the alpha subunit of EIF2 and may mediate translational control.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 protein kinase domains.
Contains 1 RWD domain.
DomainKinase domain 1 is a degenerate kinase domain.
RWD domain is reported to interact with GCN1L1.
modificationsAutophosphorylated on threonine residues.
- Information by UniProt
- E2AK4_HUMAN antibody
- Eif2ak4 antibody
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Dot blot analysis of GCN2 (phospho T899) phospho peptide (Lane 1) and GCN2 non-phospho peptide (Lane 2) labelling GCN2 (phospho T899) phospho peptide with ab75836 at a dilution of 1:1000 dilution (0.277μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST .
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75836).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab157776 has not yet been referenced specifically in any publications.