Anti-GCN5p antibody (ab63810)
Key features and details
- Rabbit polyclonal to GCN5p
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
-
Product name
Anti-GCN5p antibody -
Description
Rabbit polyclonal to GCN5p -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Recombinant fragment corresponding to the N-terminal domain (1-300aa) of Saccharomyces cerevisiae GCN5p.
-
Positive control
- Saccharomyces cerevisiae extract.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6
Preservative: 0.1% Sodium azide
Constituent: Whole serum -
Concentration information loading...
-
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).
|
Notes |
---|
WB
1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa). |
Target
-
Relevance
GCN5p is a histone acetyltransferase that contains a bromo domain and an N-acetyltransferase domain. It is recruited by a number of DNA-binding transcriptional activators, such as GCN4p and HAP2/3/4. GCN5p is a component of a number of different histone acetylation (HAT) complexes, namely, SAGA, SALSA and ADA. The SAGA complex, which is around 1.8 MDa in size, is required for the recruitment of the basal transcriptional machinery to around 10% of RNA polymerase II gene promoters in yeast. The activity of GCN5 may be dependent on its interactions within these multisubunit complexes. GCN5p acetylates histone H2B on K11 and K16 and histone H3 on K9, K14, K18, K23, K27 and K36. It also contributes to acetylation of histone H4 (at K8 and K16) and histone H2A.Z. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 853167 Saccharomyces cerevisiae
- SwissProt: Q03330 Saccharomyces cerevisiae
-
Alternative names
- ADA4 antibody
- GCN 5p antibody
- GCN5 antibody
see all
Images
-
Anti-GCN5p antibody (ab63810) at 1/500 dilution + Lysate from S. cerevisiae(BY4741)
Secondary
HRP conjugated Goat anti-Rabbit IgG at 1/5000 dilution
Predicted band size: 51 kDa -
All lanes : Anti-GCN5p antibody (ab63810) at 1/5000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Saccharomyces cerevisiae extract
Predicted band size: 51 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Additional bands at: 34 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab63810 has been referenced in 1 publication.
- Bian C et al. Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylation. EMBO J 30:2829-42 (2011). WB ; Saccharomyces cerevisiae . PubMed: 21685874