Key features and details
- Rabbit polyclonal to GCNT2
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GCNT2 antibody
DescriptionRabbit polyclonal to GCNT2
Tested applicationsSuitable for: ICC, IHC-P, WBmore details
Species reactivityReacts with: Human
antigen sequence, corresponding to amino acids 181-293 of Human GCNT2 Isoforms A and C (Q8N0V5, Q8NFS9).
- IHC-P: Human small intestine tissue section. ICC: Caco-2 cells. WB: Caco-2 and MCF-7 whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
RelevanceGCNT2 is the I-branching enzyme, a beta-1,6-N-acetylglucosaminyltransferase that converts linear into branched poly-N-acetyllactosaminoglycans and is responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding 3 different isoforms have been described.
Cellular localizationGolgi apparatus membrane.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine tissue labelling GCNT2 with ab121888 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry analysis of Caco-2 (human colorectal adenocarcinoma cell line) cells labelling GCNT2 with ab121888 at 2 µg/mL.
All lanes : Anti-GCNT2 antibody (ab121888) at 0.4 µg/ml
Lane 1 : Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Loading control: Anti-GAPDH
ab121888 has not yet been referenced specifically in any publications.