Overview

  • Product name

  • Description

    Rabbit polyclonal to GCNT3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human GCNT3 (internal sequence). The exact sequence is proprietary.
    Database link: O95395

  • Positive control

    • IHC-P: Human gastric cancer tissue. WB: A549, NCI-H1299 and HCT 116 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 51 kDa.

Target

  • Function

    Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.
  • Tissue specificity

    Primarily expressed in mucus-secreting tissues. Expressed in colon, kidney, small intestine, trachea, and stomach, where mucin is produced.
  • Pathway

    Protein modification; protein glycosylation.
  • Sequence similarities

    Belongs to the glycosyltransferase 14 family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2010013H22Rik antibody
    • 2210021I22Rik antibody
    • 2210401J11Rik antibody
    • 3-galactosyl-O-glycosyl-glycoprotein beta-1 antibody
    • 6-N-acetylglucosaminyltransferase 3 antibody
    • 6-N-acetylglucosaminyltransferase antibody
    • Beta 1 3 galactosyl O glycosyl glycoprotein beta 1 6 N acetylglucosaminyltransferase 3 antibody
    • Beta-1 antibody
    • Beta1 6 N acetylglucosaminyltransferase 3 antibody
    • beta1 6 N acetylglucosaminyltransferase antibody
    • C2/4GnT antibody
    • C24GNT antibody
    • C2GnT M antibody
    • C2GnT mucin type antibody
    • C2GnT-M antibody
    • C2GnT-mucin type antibody
    • C2GnT2 antibody
    • C2GNTM antibody
    • Core 2 beta 1 6 N acetylglucosaminyltransferase II antibody
    • Core 2/core 4 beta 1 6 N acetylglucosaminyltransferase antibody
    • Core 2/core 4 beta-1 antibody
    • dI/C2/C4GnT antibody
    • EC 2.4.1.102 antibody
    • EC 2.4.1.150 antibody
    • GCNT3 antibody
    • GCNT3_HUMAN antibody
    • Glucosaminyl (N acetyl) transferase 3 antibody
    • Glucosaminyl (N acetyl) transferase 3 mucin type antibody
    • glucosaminyl (N-acetyl) transferase 3, mucin type antibody
    • GnT M antibody
    • GNTM antibody
    • hC2GnT M antibody
    • hC2GnT-M antibody
    • Mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase antibody
    • OTTHUMP00000163601 antibody
    see all

Images

  • All lanes : Anti-GCNT3 antibody (ab227972) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
    Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 51 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded human gastric cancer tissue stained for GCNT3 using ab227972 at 1/500 dilution in immunohistochemical analysis.

References

ab227972 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227972.
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