Overview

  • Product name

  • Description

    Goat polyclonal to GCNT3
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Cow, Human
    Predicted to work with: Mouse, Rat, Horse, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    C-NKFDPKVDDNALQ

    , corresponding to internal sequence amino acids 410-422 of Human GCNT3 (NP_004742.1).

  • Positive control

    • Human Duodenum lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab77728 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 51 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.
    • Tissue specificity

      Primarily expressed in mucus-secreting tissues. Expressed in colon, kidney, small intestine, trachea, and stomach, where mucin is produced.
    • Pathway

      Protein modification; protein glycosylation.
    • Sequence similarities

      Belongs to the glycosyltransferase 14 family.
    • Post-translational
      modifications

      N-glycosylated.
    • Cellular localization

      Golgi apparatus membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2010013H22Rik antibody
      • 2210021I22Rik antibody
      • 2210401J11Rik antibody
      • 3-galactosyl-O-glycosyl-glycoprotein beta-1 antibody
      • 6-N-acetylglucosaminyltransferase 3 antibody
      • 6-N-acetylglucosaminyltransferase antibody
      • Beta 1 3 galactosyl O glycosyl glycoprotein beta 1 6 N acetylglucosaminyltransferase 3 antibody
      • Beta-1 antibody
      • Beta1 6 N acetylglucosaminyltransferase 3 antibody
      • beta1 6 N acetylglucosaminyltransferase antibody
      • C2/4GnT antibody
      • C24GNT antibody
      • C2GnT M antibody
      • C2GnT mucin type antibody
      • C2GnT-M antibody
      • C2GnT-mucin type antibody
      • C2GnT2 antibody
      • C2GNTM antibody
      • Core 2 beta 1 6 N acetylglucosaminyltransferase II antibody
      • Core 2/core 4 beta 1 6 N acetylglucosaminyltransferase antibody
      • Core 2/core 4 beta-1 antibody
      • dI/C2/C4GnT antibody
      • EC 2.4.1.102 antibody
      • EC 2.4.1.150 antibody
      • GCNT3 antibody
      • GCNT3_HUMAN antibody
      • Glucosaminyl (N acetyl) transferase 3 antibody
      • Glucosaminyl (N acetyl) transferase 3 mucin type antibody
      • glucosaminyl (N-acetyl) transferase 3, mucin type antibody
      • GnT M antibody
      • GNTM antibody
      • hC2GnT M antibody
      • hC2GnT-M antibody
      • Mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase antibody
      • OTTHUMP00000163601 antibody
      see all

    Images

    References

    This product has been referenced in:

    • Li RW  et al. The vitamin D receptor and inducible nitric oxide synthase associated pathways in acquired resistance to Cooperia oncophora infection in cattle. Vet Res 42:48 (2011). WB ; Cow . Read more (PubMed: 21414188) »
    See 1 Publication for this product

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