Key features and details
- Goat polyclonal to GCNT3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GCNT3 antibody
See all GCNT3 primary antibodies
DescriptionGoat polyclonal to GCNT3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab77728 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab77728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 51 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionGlycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.
Tissue specificityPrimarily expressed in mucus-secreting tissues. Expressed in colon, kidney, small intestine, trachea, and stomach, where mucin is produced.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the glycosyltransferase 14 family.
Cellular localizationGolgi apparatus membrane.
- Information by UniProt
- 2010013H22Rik antibody
- 2210021I22Rik antibody
- 2210401J11Rik antibody
Anti-GCNT3 antibody (ab77728) at 0.1 µg/ml + Human Duodenum lysate (in RIPA buffer) at 35 µg
Predicted band size: 51 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
ab77728 has been referenced in 1 publication.
- Li RW et al. The vitamin D receptor and inducible nitric oxide synthase associated pathways in acquired resistance to Cooperia oncophora infection in cattle. Vet Res 42:48 (2011). WB ; Cow . PubMed: 21414188