Key features and details
- Rabbit polyclonal to GCP4
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-GCP4 antibody
See all GCP4 primary antibodies
DescriptionRabbit polyclonal to GCP4
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
Recombinant fragment, corresponding to a region within amino acids 99-314 of Human GCP4 (UniProt: Q9UGJ1).
- NIH 3T3 whole cell lysate; 293T cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab153939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 76 kDa.|
|ICC/IF||1/100 - 1/1000.|
RelevanceGCP4 is a component of the gamma-tubulin complex which is necessary for microtubule nucleation at the centrosome. GCP4 shows sequence identity with the C-terminal regions of human centrosomal proteins 103p (TUBGCP2) and 104p (TUBGCP3).
- 76P antibody
- FLJ14797 antibody
- Gamma tubulin complex component 4 antibody
Anti-GCP4 antibody (ab153939) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 76 kDa
10 % SDS-PAGE
Confocal Immunofluorescent analysis of methanol-fixed 293T cells labeling GCP4 with ab153939 at 1/500 dilution (green). Alpha-tubulin filaments are labeled in red
Confocal Immunofluorescent analysis of paraformaldehyde-fixed 293T cells labeling GCP4 with ab153939 at 1/500 dilution (green). Alpha-tubulin filaments are labeled in red
ab153939 has not yet been referenced specifically in any publications.