Anti-GCP6 antibody (ab95172)
Key features and details
- Rabbit polyclonal to GCP6
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GCP6 antibody -
Description
Rabbit polyclonal to GCP6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan -
Immunogen
Synthetic peptide corresponding to Human GCP6 aa 1350-1450.
Database link: Q96RT7 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 200 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 200 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Relevance
The protein encoded by this gene is part of a large multisubunit complex required for microtubule nucleation at the centrosome. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 85378 Human
- Omim: 610053 Human
- SwissProt: Q96RT7 Human
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Alternative names
- Gamma tubulin complex component 6 antibody
- GCP 6 antibody
- MCCRP antibody
see all
Images
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All lanes : Anti-GCP6 antibody (ab95172) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 200 kDa -
Lane 1: 3µg ab95172 were used to immunoprecipitate GCP3 from 1mg HeLa whole cell lysate. 20% IP was loaded per lane and probed with ab95170 at 1µg/ml.
Lane 2: same, using a control IgG.
Detection: chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab95172 has been referenced in 6 publications.
- Shankar S et al. Α γ-tubulin complex-dependent pathway suppresses ciliogenesis by promoting cilia disassembly. Cell Rep 41:111642 (2022). PubMed: 36384111
- Lindström L & Alvarado-Kristensson M Characterization of gamma-tubulin filaments in mammalian cells. Biochim Biophys Acta 1865:158-171 (2018). WB . PubMed: 29050966
- Cota RR et al. MZT1 regulates microtubule nucleation by linking ?TuRC assembly to adapter-mediated targeting and activation. J Cell Sci 130:406-419 (2017). PubMed: 27852835
- Farache D et al. Functional Analysis of ?-Tubulin Complex Proteins Indicates Specific Lateral Association via Their N-terminal Domains. J Biol Chem 291:23112-23125 (2016). PubMed: 27660388
- Lecland N & Lüders J The dynamics of microtubule minus ends in the human mitotic spindle. Nat Cell Biol 16:770-8 (2014). PubMed: 24976384
- Singh P et al. TACC3 protein regulates microtubule nucleation by affecting ?-tubulin ring complexes. J Biol Chem 289:31719-35 (2014). PubMed: 25246530