Anti-GCSF Receptor antibody [3G10G2] (ab233833)
Key features and details
- Mouse monoclonal [3G10G2] to GCSF Receptor
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-GCSF Receptor antibody [3G10G2]
See all GCSF Receptor primary antibodies -
Description
Mouse monoclonal [3G10G2] to GCSF Receptor -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human GCSF Receptor aa 1-200 (extracellular). Expressed in E.coli.
Database link: Q99062 -
Positive control
- WB: Recombinant human GCSF Receptor (aa 25-187) protein; GCSF Receptor (aa 25-187)-hIgGFc-transfected HEK-293 cell lysate. Flow cyt: K562 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3G10G2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000.
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Flow Cyt |
1/200 - 1/400.
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Notes |
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WB
1/500 - 1/2000. |
Flow Cyt
1/200 - 1/400. |
Target
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Function
Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface. -
Tissue specificity
One or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U-937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U-937 cells. Isoform GCSFR-3 is highly expressed in placenta. -
Involvement in disease
Hereditary neutrophilia -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1441 Human
- Omim: 138971 Human
- SwissProt: Q99062 Human
- Unigene: 524517 Human
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Alternative names
- CD 114 antibody
- CD114 antibody
- CD114 antigen antibody
see all
Images
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Anti-GCSF Receptor antibody [3G10G2] (ab233833) at 1/100 dilution + Recombinant human GCSF Receptor (aa 25-187) protein
Expected recombinant MW is 47 kDa.
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All lanes : Anti-GCSF Receptor antibody [3G10G2] (ab233833) at 1/100 dilution
Lane 1 : Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : GCSF Receptor (aa 25-187)-hIgGFc-transfected HEK-293 cell lysate -
Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling GCSF Receptor with ab233833 at 1/200 dilution (green) compared to a negative control (red).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab233833 has been referenced in 1 publication.
- Li F et al. Jiyuan oridonin A induces differentiation of acute myeloid leukemia cells including leukemic stem-like cells. Front Pharmacol 13:1001552 (2022). PubMed: 36133825