Overview

  • Product name

    Anti-GCSF Receptor antibody
    See all GCSF Receptor primary antibodies
  • Description

    Rabbit polyclonal to GCSF Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 164-392 of Human GCSF Receptor (UniProt Q99062).

  • Positive control

    • CL1-5 xenograft; A431 cells; A431 whole cell lysate; 293T and Raji

Properties

Applications

Our Abpromise guarantee covers the use of ab126167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 92 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Function

    Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface.
  • Tissue specificity

    One or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U-937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U-937 cells. Isoform GCSFR-3 is highly expressed in placenta.
  • Involvement in disease

    Hereditary neutrophilia
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 5 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 114 antibody
    • CD114 antibody
    • CD114 antigen antibody
    • Colony stimulating factor 3 receptor (granulocyte) antibody
    • Colony stimulating factor 3 receptor antibody
    • CSF 3R antibody
    • CSF3R antibody
    • CSF3R_HUMAN antibody
    • Csfgr antibody
    • G CSF R antibody
    • G-CSF receptor antibody
    • G-CSF-R antibody
    • GCSFR antibody
    • Granulocyte colony stimulating factor receptor antibody
    • Granulocyte colony-stimulating factor receptor antibody
    • OTTHUMP00000009703 antibody
    • OTTHUMP00000009704 antibody
    • OTTHUMP00000009705 antibody
    see all

Images

  • Anti-GCSF Receptor antibody (ab126167) at 1/1000 dilution + A431 whole cell lysate
    at 30 µg

    Predicted band size: 92 kDa



    7.5% SDS PAGE
  • ab126167, at 1/500 dilution, staining GCSF Receptor in methanol-fixed A431 by Immunonfluorescence. The lower image is co-stained with Hoechst 33342.
  • ab126167, at 1/250 dilution, staining GCSF Receptor in paraffin-embedded CL1-5 xenograft by Immunohistochemistry.

References

This product has been referenced in:

  • Dwivedi P  et al. Phospho serine and threonine analysis of normal and mutated granulocyte colony stimulating factor receptors. Sci Data 6:21 (2019). Read more (PubMed: 30967555) »
  • Zhang H  et al. Unpaired Extracellular Cysteine Mutations of CSF3R Mediate Gain or Loss of Function. Cancer Res 77:4258-4267 (2017). Read more (PubMed: 28652245) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. I have been able to find your order by searching the PO number.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1185453.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.


I wish you the best of luck with your research.

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Answer

Thank you for taking time to contacting us. I am sorry to hear this antibody is not providing satisfactory results.


The details which you provide during our conversation will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed. Please include your Abcam order confirmation number so that I might process the replacement, credit note or refund for you.

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