Product nameAnti-GCSF Receptor antibody [EPR8957]
See all GCSF Receptor primary antibodies
DescriptionRabbit monoclonal [EPR8957] to GCSF Receptor
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) to residues in the intracellular domain of Human GCSF Receptor. (Q99062).
- Human placenta extract, NCCIT cell lysate, Caco-2 cell lysate
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab156878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 92 kDa.|
|Flow Cyt||1/500 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionReceptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface.
Tissue specificityOne or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U-937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U-937 cells. Isoform GCSFR-3 is highly expressed in placenta.
Involvement in diseaseHereditary neutrophilia
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD 114 antibody
- CD114 antibody
- CD114 antigen antibody
Flow cytometric analysis of permeabilized NCCIT cells labeling GCSF Receptor using ab156878 antibody at a dilution of 1/500 (red) or a rabbit IgG (negative) (green).
All lanes : Anti-GCSF Receptor antibody [EPR8957] (ab156878) at 1/1000 dilution
Lane 1 : Human placenta extract
Lane 2 : NCCIT cell lysate
Lane 3 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 92 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
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This product has been referenced in:
- Wu Y et al. Expression and role of granulocyte macrophage colony-stimulating factor receptor (GM-CSFR) and granulocyte colony-stimulating factor receptor (G-CSFR) on Ph-positive acute B lymphoblastic leukemia. Hematology N/A:1-9 (2018). Human . Read more (PubMed: 29338593) »