Anti-GDF 9 antibody (ab38544)
Key features and details
- Rabbit polyclonal to GDF 9
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-GDF 9 antibody
See all GDF 9 primary antibodies -
Description
Rabbit polyclonal to GDF 9 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
A KLH conjugated synthetic peptide (10-30 aa in length) at the N-term of first 60 aa of human GDF 9.
-
Positive control
- HL60 cell lysate and hepatocarcinoma tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab38544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/100.
|
|
WB |
1/100 - 1/500. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
|
Notes |
---|
IHC-P
1/50 - 1/100. |
WB
1/100 - 1/500. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
Target
-
Relevance
GDF 9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Growth factors synthesized by ovarian somatic cells directly affect oocyte growth and function. GDF 9 is expressed in oocytes and is thought to be required for ovarian folliculogenesis. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 2661 Human
- Omim: 601918 Human
- SwissProt: O60383 Human
-
Alternative names
- GDF9 antibody
- Growth differentiation factor 9 antibody
Images
-
Anti-GDF 9 antibody (ab38544) at 1/100 dilution + HL60 cell lysate
Predicted band size: 51 kDa
Observed band size: 51 kDa -
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab38544 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab38544 has been referenced in 2 publications.
- Hu X et al. Stem cells derived from human first-trimester umbilical cord have the potential to differentiate into oocyte-like cells in vitro. Int J Mol Med 35:1219-29 (2015). PubMed: 25760093
- Wang Y et al. Extra-ovarian expression and activity of growth differentiation factor 9. J Endocrinol 202:419-30 (2009). PubMed: 19505950