Recombinant
RabMAb

Recombinant Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)

Overview

  • Product name

    Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)]
    See all GDF11 + GDF8/Myostatin primary antibodies
  • Description

    Rabbit monoclonal [EPR4567(2)] to GDF11 + GDF8/Myostatin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human GDF11 + GDF8/Myostatin aa 350 to the C-terminus.
    Database link: O95390

  • Positive control

    • WB: HeLa, HEK293 and C6 cell lysate and human heart tissue lysate. C2C12 cell lysate. IHC-P: Human colon adenocarcinoma and cerebral cortex tissues. ICC/IF: A549 cells.
  • General notes

     

     

     This product was previously labelled as BMP11, BMP11 + GDF8 / Myostatin

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Purified format.

ICC/IF 1/250.
WB 1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/500 - 1/1000.

Target

Images

  • Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721) at 1/1000 dilution + C2C12 (mouse myoblast) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.



    Blocking and Diluting buffer: 5% NFDM /TBST

  • Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721) at 1/1000 dilution (purified) + HEK293 cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon adenocarcinoma tissue labelling BMP11 + GDF8/Myostatin with unpurified ab124721 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • ab124721 staining GDF11 + GDF8/Myostatin in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721) at 1/5000 dilution (purified) + HeLa cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721) at 1/1000 dilution (purified) + C6 cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Human heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling BMP11 + GDF8/Myostatin with purified ab124721 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/Immunofluorescence analysis of A549 cells labelling BMP11 + GDF8/Myostatin with purified ab124721 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. ab150078, an Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/250) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:

  • Tito A  et al. The Growth Differentiation Factor 11 is Involved in Skin Fibroblast Ageing and is Induced by a Preparation of Peptides and Sugars Derived from Plant Cell Cultures. Mol Biotechnol 61:209-220 (2019). Read more (PubMed: 30661170) »
  • Gai J  et al. Contrast-enhanced computed tomography combined with Chitosan-Fe3O4 nanoparticles targeting fibroblast growth factor receptor and vascular endothelial growth factor receptor in the screening of early esophageal cancer. Exp Ther Med 15:5344-5352 (2018). Read more (PubMed: 29805549) »
See all 15 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

1-6 of 6 Abreviews

Application
ELISA
Sample
Mouse Serum (heparin tubes collected whole blood and then spun)
Specification
heparin tubes collected whole blood and then spun
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Type
Sandwich (Capture)

Abcam user community

Verified customer

Submitted Sep 14 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
liver
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 20% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 13 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse liver + kidney + heart & human Hela cells)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
100 µg
Treatment
Halt Phosphatase inhibitor cocktail before lysis
Specification
Mouse liver + kidney + heart & human Hela cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Selina Moebus

Verified customer

Submitted May 28 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Skin and DRG in situ)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Skin and DRG in situ
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Jul 25 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Jul 25 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Testis
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Jul 25 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up