Recombinant
RabMAb

Recombinant Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)

Overview

  • Product name

    Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)]
    See all GDF11 + GDF8/Myostatin primary antibodies
  • Description

    Rabbit monoclonal [EPR4567(2)] to GDF11 + GDF8/Myostatin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human GDF11 + GDF8/Myostatin aa 350 to the C-terminus.
    Database link: O95390

  • Positive control

    • WB: HeLa, HEK293 and C6 cell lysate and human heart tissue lysate. C2C12 cell lysate. IHC-P: Human colon adenocarcinoma and cerebral cortex tissues. ICC/IF: A549 cells.
  • General notes

     

     

     This product was previously labelled as BMP11, BMP11 + GDF8 / Myostatin

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Purified format.

ICC/IF 1/250.
WB 1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/500 - 1/1000.

Target

Images

  • Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721) at 1/1000 dilution + C2C12 (mouse myoblast) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.



    Blocking and Diluting buffer: 5% NFDM /TBST

  • Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721) at 1/1000 dilution (purified) + HEK293 cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon adenocarcinoma tissue labelling BMP11 + GDF8/Myostatin with unpurified ab124721 at 1/500 dilution.

  • ab124721 staining GDF11 + GDF8/Myostatin in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721) at 1/5000 dilution (purified) + HeLa cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721) at 1/1000 dilution (purified) + C6 cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Human heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling BMP11 + GDF8/Myostatin with purified ab124721 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/Immunofluorescence analysis of A549 cells labelling BMP11 + GDF8/Myostatin with purified ab124721 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. ab150078, an Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/250) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:

  • Tito A  et al. The Growth Differentiation Factor 11 is Involved in Skin Fibroblast Ageing and is Induced by a Preparation of Peptides and Sugars Derived from Plant Cell Cultures. Mol Biotechnol 61:209-220 (2019). Read more (PubMed: 30661170) »
  • Gai J  et al. Contrast-enhanced computed tomography combined with Chitosan-Fe3O4 nanoparticles targeting fibroblast growth factor receptor and vascular endothelial growth factor receptor in the screening of early esophageal cancer. Exp Ther Med 15:5344-5352 (2018). Read more (PubMed: 29805549) »
See all 15 Publications for this product

Customer reviews and Q&As

1-6 of 6 Q&A

Answer


I have compared the immunogen of ab124721 with myostatin and there is an 80% homology so there are chances that this antibodies also recognises Myostatin however, we have not specifically tested this so can't be sure.
I have compared the total GDF11 protein sequence with the Myostatin sequence and the C terminus of these two proteins is very similar. The N terminus however, it quite different and so if you wanted an antibody that recognises GDF11 only, an aminoterminal antibody such as ab71347 might be better.

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Answer

Thank you for contacting us. We don't determine the exact MW of the antibodies themselves, but I can ell you that antibodies are generally 140 kD.

The MW of the target BMP11 is 45 Kd, which is the size it has come up on our WB.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your reply. Yes, we do recommend storing this antibody at -20C and it should be stable for at least 12 months from the date of purchase. Please let me know if you need any additional information or assistance.

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Answer

Merci de votre émail.

Nous n'avons pas encore testé si le ab71347 peux réagir avec la séquence de souris. En regardant l'alignement de la séquence de la protéine humaine avec la séquence de la protéine de souris, il me semble fort probable que le ab71347 peux réagir avec la protéine de souris. http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20120514-105011-0843-68673285-oy

Néanmoins, comme à notre connaissance, cet anticorps n’a pas encore été testéchez la souriset nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires, il me sera possible de vous offrir une remise si vous souhaitez tester ab71347 chez la souris: Après soumission d’une Abreview avec vos résultats, une remise correspondant à la valeur d’achat de ab71347 pourra être utilisable lors d’une prochaine commande d’anticorps primaire.

Etapes à suivre :

1. Nous confirmer que vous souhaiteriez tester ab71347 chez la sourisafin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab71347

2. Acheter ab71347 par téléphone, fax ou internet (www.abcam.com)

3. Le tester chez la souris

4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews

5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire.
Même si ab71347 est démontré ne pas fonctionner chez la souris, après soumission de votre Abreview, vous recevrez quand même une remise sur l’achat de votre prochain anticorps.

N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.

Termes et Conditions : www.abcam.com/collaborationdiscount.

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Answer

Merci de nous avoir contactés.

Nous vous envoyé le devis. Par contre je dois vous informer que le ab124721 n'as pas encore été testé en ELISA. Il se peux qu'il fonctionne, mais comme nous avons eu du feedback que cet anticorps ne fonctionne pas en immunoprécipitation ou en cytométrie de flux, je ne suis pas très optimiste que cet anticorps peux fonctionner en ELISA.

Nous avons l'anticorps ab71347 qui est testé et garantie par nous de fonctionner en ELISA. Est-ce que cet anticorps peux vous intéresser? https://www.abcam.com/index.html?datasheet=71347 (or use the following: https://www.abcam.com/index.html?datasheet=71347).

Veuillez ne pas hésiter à nous demander plus de conseil ou de renseignement pour le choix d'anticorps.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Answer

Thank you for contacting us.

I have gathered the following information:


ab124721: The immunogen range is located within the mature human protein (aa 299 - 407).


ab56645:immunogne is a recombinant fragment: ESRCCRYPLT VDFEAFGWDW IIAPKRYKAN YCSGQCEYMF MQKYPHTHLV QQANPRGSAG PCCTPTKMSP INMLYFNDKQ QIIYGKIPGM VVDRCGCS, corresponding to amino acids 310-408 of Human BMP11. Thus, it binds to the mature protein.


ab71347: The immunogen sequenceis located outside the mature human protein (aa 299 – 407), further N-terminalof thehuman protein.


human protein: Signal peptide aa1 – 24; Propeptide aa25 – 298; mature Chain aa299 – 407
mouse protein: Signal peptide aa1 – 20; Propeptide aa21 – 296; mature Chain aa297 – 405


Please see also http://www.uniprot.org/uniprot/O95390for the human protein and http://www.uniprot.org/uniprot/Q9Z1W4for the mouse protein for the processing of the protein in each species.

The homology between human and mouse protein is 99%. Thus, if you would like to try any of the above antibodies inmouse (except ab124721 which has been tested already in mouse), you can use our testing discount program.


For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.


Please let me know if I shall send you a testing code and if so for which antibody.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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