Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)]

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Testis
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/2000

Secondary antibody

Dilution
1/250
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
For rating, I would rather use "Uncertain" than "Inconclusive" as I have no knowledge of the distribution pattern of this protein in tissue sections nor can I find published images. Submitted image of adult testis shows a single large positive area in Sertoli cells ( black arrowhead) similar in developing sperm cells ( red arrowhead). In the latter many cells show multiple positive dots ( green arrowhead). In the Sertoli cells there is also a moderate diffuse cytoplasmic positivity.
Abcam response
Thank you for your valuable feedback. We were able to find evidence of BMP11 mRNA expression in the testis, but were unable to verify protein distribution in this tissue. We welcome further customer comments. For more information, please contact scientific support.

Mr. Carl Hobbs

Verified customer

Submitted Jul 25 2013

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