Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BMP11 antibody [EPR4567(2)]

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/3000

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250

Additional data

Additional Notes
Submitted image of adult mouse cortex shows a distinct punctate positivity in neurons ( maybe in other cells, also). In human, mouse and rat this positivity appears similar is it Golgi Apparatus that is positive? Is it specific?
Abcam response
Thank you for your valuable feedback. We were able to find evidence of low BMP11 mRNA expression in the brain, but were unable to verify protein distribution in this tissue. We welcome further customer comments. For more information, please contact scientific support.

Mr. Carl Hobbs

Verified customer

Submitted Jul 25 2013

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