Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)]

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Skin and DRG in situ)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Skin and DRG in situ
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/3000

Secondary antibody

Dilution
1/250
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
This Ab seems to give the same pattern of positivity in rat, mouse and human tissues. However, as I do not know of the /cannot find images showing localisation of BMP11 in tissue sections, I would rather rate my results "uncertain". Submitted composite image of rat e17 skin ( upper) and DRG ( lower) shows two distinct patterns of positivity. One is a distinct single "granule" in seemingly all cells ( most prominent in DRG neurons) the other is the basal lamina positivity seen in the skin. Note that this area is negative in the developing hair follicle shown.
Abcam response
Thank you for your valuable feedback. We were able to find evidence of BMP11 mRNA expression in keratinocytes and the spinal cord, but were unable to verify protein distribution in these tissues. We welcome further customer comments. For more information, please contact scientific support.

Mr. Carl Hobbs

Verified customer

Submitted Jul 25 2013

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